Intensity Test - Agilent Technologies 1100 Series Reference Manual

3 Troubleshooting and Test Functions
• Dirty or contaminated optical components (achromat, windows).
• Old or non-Agilent lamp.
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Intensity Test

The test ist for the standard flow cells (10 mm and 6 mm pathlength) only. The nano-
NOTE
flow cells (80 nl and 500 nl) cannot be run with this test due to its low volume.
The intensity test measures the intensity of the deuterium and tungsten lamps over the
full wavelength range (190 – 950 nm). Four spectral ranges are used to evaluate the
intensity spectrum. The test is used to determine the performance of the lamps and optics
(see also
light path automatically, and the gain is set to zero. To eliminate effects due to absorbing
solvents, the test should be done with water in the flow cell. The shape of the intensity
spectrum is primarily dependent on the lamp, grating, and diode array characteristics.
Therefore, intensity spectra will differ slightly between instruments.
typical intensity test spectrum.
78 1100 Series DAD and MWD Reference Manual
Suggested Actions
Ensure the flow cell is filled with water.
"Wavelength Recalibration"
Recalibrate (see
Run the cell test (see
windows.
Clean optical components with alcohol and lint-free cloth.
Exchange the UV lamp.
"Cell Test"
on page 90). When the test is started, the 1-nm slit is moved into the
"Cell Test"
on page 90). If the test fails, exchange the flow cell
on page 84) and repeat the test.
Figure 23 shows a