Agilent Technologies 1100 Series Reference Manual page 294

A Safety Information
Avoid the use of alkaline solutions (pH > 9.5) which can attack quartz and thus impair
the optical properties of the flow cell.
Prevent any crystallization of buffer solutions. This will lead into a blockage/damage of
the flow cell.
If the flow cell is transported while temperatures are below 5 degree C, it must be
assured that the cell is filled with alcohol.
Aqueous solvents in the flow cell can built up algae. Therefore do not leave aqueous
solvents sitting in the flow cell. Add small % of organic solvents (e.g. Acetonitrile or
Methanol ~5%).
Solvents
Brown glass ware can avoid growth of algae.
Always filter solvents, small particles can permanently block the capillaries. Avoid the
use of the following steel-corrosive solvents:
• Solutions of alkali halides and their respective acids (for example, lithium iodide,
• High concentrations of inorganic acids like nitric acid, sulfuric acid especially at
• Halogenated solvents or mixtures which form radicals and/or acids, for example:
• Chromatographic grade ethers, which can contain peroxides (for example, THF,
• Solutions of organic acids (acetic acid, formic acid, and so on) in organic solvents.
• Solutions containing strong complexing agents (for example, EDTA, ethylene
• Mixtures of carbon tetrachloride with 2-propanol or THF.
294 1100 Series DAD and MWD Reference Manual
potassium chloride, and so on).
higher temperatures (replace, if your chromatography method allows, by phosphoric
acid or phosphate buffer which are less corrosive against stainless steel).
→ 2COCl
2CHCl + O
3 2
This reaction, in which stainless steel probably acts as a catalyst, occurs quickly with
dried chloroform if the drying process removes the stabilizing alcohol.
dioxane, di-isopropylether) such ethers should be filtered through dry aluminium
oxide which adsorbs the peroxides.
For example, a 1-% solution of acetic acid in methanol will attack steel.
diamine tetra-acetic acid).
+ 2HCl
2