Getinge Applikon my-Control Hardware Manual page 55

In case the assembly is used for addition, pump tubing can be used to connect
the Addition Vial dip tube with the addition port of the MiniBio Reactor.
Before autoclaving, the vial is filled with addition liquid.
After autoclaving, the pump tubing can be inserted in the tubing pump at the my-
Control and the addition assembly will be ready to be used.
The assembly can also be used for sampling.
In this case, the dip tube in the vial has to be cut off (approx.. 1 cm below the
bottom of the cap).
Connect a syringe to the air filter (using a piece of additional tubing).
Connect the sample tube side of the assembly with the sample pipe in the
MiniBio Reactor.
Before autoclaving, the vial is empty.
During cultivation, the sampling system can be operated using the syringe:
• Air from the syringe can be forced though the filter to empty the sample tube
• Afterwards, sample can be drawn through the sample pipe and tubing into
the sample vial.
• The sampling action must be completed by using the syringe to force the
content of the sample tubing back into the reactor.
After sampling, the filled sample vial can be replaced by a (sterile) new one in a
sanitary manner.
Hardware Manual my-Control MiniBio
REACTORS AND AUXILIARIES
55