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6.3 MiniBio Reactor Assembly 1000 ml .............................................................................................. 39
6.3.1 General Reactor Specifications ........................................................................................ 39
6.3.2 Head Plate Specifications ................................................................................................. 40
6.3.3 Required Space in Autoclave ........................................................................................... 40
6.3.4 Bioreactor Assembly 1000 ml .......................................................................................... 41
6.4 Stirrer Drive and Stirrer Assembly ................................................................................................ 43
6.4.1 Impellers ........................................................................................................................... 44
6.4.2 Maximum Stirrer Speed ................................................................................................... 44
6.4.3 Maximum Torque of the Stepper Stirrer Motors .............................................................. 45
6.4.4 Advised Impeller Configuration ....................................................................................... 46
6.4.5 Stirrer Power calculations and examples .......................................................................... 47
6.5 Off-gas Line Configuration ............................................................................................................ 49
6.5.1 Cell Culture Applications ................................................................................................. 49
6.5.2 Microbial Applications ..................................................................................................... 49
6.5.3 the Condenser on the stand of the 250 and 500 ml Reactor ............................................. 50
6.5.4 the Condenser on the HEad Plate of the 1000 ml Reactor ............................................... 51
6.5.5 Gas Inlet / Outlet Filters ................................................................................................... 52
6.6 Sample Pipe (adj. height) with Luer Lock Connection .................................................................. 52
6.7 Septum Assembly .......................................................................................................................... 52
6.8 Clamping Nuts and Blind Nuts ...................................................................................................... 53
6.9 Tools for fastening the Nuts ........................................................................................................... 53
6.10 Liquid Addition / Sampling Assembly (250 and 500 ml MiniBio Reactor) .................................. 54
6.11 Liquid Addition Bottle and Bottle Holder for 1000ml MiniBio Reactor ....................................... 56
6.12 Single Use Sample System............................................................................................................. 57
6.13 Sensors ........................................................................................................................................... 58
6.13.1 pH and DO Sensors .......................................................................................................... 58
6.13.2 Level Sensors ................................................................................................................... 58
6.13.3 Temperature Sensor .......................................................................................................... 59
6.13.4 USB Sensors ..................................................................................................................... 59
6.14 Start-Up Kits .................................................................................................................................. 60
6.14.1 Start-up Kit 250 ml MiniBio Reactor ............................................................................... 60
6.14.2 Start-up Kit 500 ml MiniBio Reactor ............................................................................... 61
6.14.3 Start-up Kit 1000 ml MiniBio Reactor ............................................................................. 62
Hardware Manual my-Control MiniBio
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