Thermo Scientific VC-D60 Operating Manual page 91

Preparations
Cleaning procedure for organic solvent
Binding of proteins, salts, or sugars
Heavy-duty cleaning
Refractive Index Detector (VC-D60)
Operating Manual
To introduce the cleaning solution into the detector, connect an inlet
capillary directly from the pump to the flow cell inlet port, bypassing the
column. For safety, make sure to connect a waste tubing to the outlet.
Some cleaning solutions, however, should be injected directly into the
flow cell using a syringe due to their high corrosiveness or safety
concerns. You can inject these solutions by using the optional flushing
and injection kit.
TIP Clean all internal lines of the detector by injecting cleaning solution
with PURGE Off, and inject cleaning solution again with PURGE On.
1. Disconnect the inlet capillary and press PURGE to turn on the valve.
2. By syringe, inject 30 to 50 mL of solvents (in order of acetone, THF,
chloroform, methanol and acetone).
3. Press PURGE every 10 seconds to on/off the purge valve while
injecting those solvents.
4. As an alternative, fill the flow cell(s) with acetone and leave it
overnight.
Repeat the procedure if the result is not satisfactory.
1. Connect detector with contamination free solvent delivery pump.
2. Press PURGE to turn on the valve.
3. Pump deionized water at 1mL/min flow rate and leave it overnight.
4. You may want to wash the flow path with 0.1M NaOH priory if you
know there is a potential of proteins binding.
Repeat the procedure if the result is not satisfactory.
If the above whole procedure didn't work, you may apply 15% nitric acid
solution. Make sure to flush the flow path with deionized water before
and after.
Apply the nitric acid solution using a syringe and make sure the solution
does not stay more than 5 minutes.
7 • Maintenance and Service
Page 91