Operating Instructions; Starting Up - Filling The Humidifier - Thermo Scientific Vitrobot Mk IV User Manual

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Chapter | Operating Instructions
3

Operating Instructions

In this chapter, the complete operation of the Vitrobot, both hardware and software is
stepwise explained and where possible visualized.
3.1 Starting Up – Filling the Humidifier
Prior to activation of the Vitrobot, the humidifier must be filled with distilled water. For this, the
syringe needs to be filled with 60 ml of water before filling the humidifier through the plastic
tube at the bottom part. After injection of the water into the humidifier, it is important that an
"under-vacuum" is created inside the syringe (by "de-filling or pulling" the syringe while
keeping attached to the plastic tube). By pulling out "air" from the humidifier, the humidifier is
properly filled with water (see also the instruction movie for further details).
The humidifier is bajonet-attached to the bottom of the climate chamber. By turning and
pulling, the humidifier can be removed. For further details on the exchange or refreshment of
the water see:
on page
30
Confidential, limited rights
PN 103261 | Revision D | 23-Oct-2019
User Manual
Page 16

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