Technical Specification; Key Features - Thermo Scientific Vitrobot Mk IV User Manual

Chapter | Introduction

1.3 Technical Specification

1.3.1 Key Features

•
Temperature: software controlled, direct read out, and range: 4 - 60C. Peltier type of
heating/cooling.
•
Humidity: software controlled, direct read out, and range: room conditions up to 100 % rH.
Ultrasonic humidifier.
•
Sample application: software controlled dipping/withdrawing from a vial. Small sample
volumes can be applied manually through a side port allowing access to a pipette. The
time between sample application and blotting ("wait time") is controlled by software.
•
Blotting: removal of excess liquid by a controlled blotting action using filter paper on
(rotating) foam pads. The number of blotting actions (1 - 16) as well as the duration of the
blotting action is software controlled. Time between blotting and vitrification ("drain time")
can be set.
•
Vitrification: smooth acceleration of the thin film to the liquid ethane container. A shutter
opens the chamber automatically to give the specimen access to the liquid/solid ethane
(-178C). Tailored damping action prevents bouncing of the specimen during v itrification.
A lift for the ethane container brings the coolant as close as possible to the shutter to
ensure optimal vitrification. The carriage of the vitrified specimen is coupled to the ethane
lift and by lowering this assembly the vitrified specimen can be retrieved and stored for
further investigations.
•
Process control: the entire vitrification process is controlled by a built in PC. Dedicated
software is used for process control and this program allows tailoring of relevant
parameters to fit the specimen and the design of experiments.
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PN 103261 | Revision D | 23-Oct-2019
User Manual
Page 7