Thermo Scientific Vitrobot Mk IV User Manual page 21

Chapter | Operating Instructions
In the Miscellaneous box, the use of a foot pedal switch (as alternative for the stylus on the
touchscreen), and the possibility to switch off the humidifier during manual application and
plunge freezing can be chosen. The semi-automatic grid transfer (i.e. the automatic
movement of the grid from the liquid ethane/propane towards the grid box in the liquid
nitrogen atmosphere) is default activated but can be de-activated by checking the skip box. In
case the auto raise ethane lift option is checked, the coolant container will be lifted towards
the bottom of the climate chamber simultaneously with the uplift of the tweezer (you can also
do this in separate actions).
In addition, all essential freezing parameters can be saved ('Save') and loaded ('Load')
according to the type of sample or experiment that is performed.
Summary of Parameters that can be set
Blot-time (s)
Blot process
Blot force
Drain time (s)
Wait time (s)
Skip application process
Use foot pedal
Hum off during process
Skip Grid Transfer
Auto raise Ethane lift
Confidential, limited rights
PN 103261 | Revision D | 23-Oct-2019
Parameter Range
0 - 99999 ± 0.1
0 - 16 1
-25 - +25 ± 1
0 - 99999 ± 0.1
0 - 99999 ± 0.1
on / off
on / off
on / off ransfer
on / off
on / off
Increment
± 0.5
1
± 1
± 0.5
± 0.5
User Manual
Page 21