Thermo Scientific Vitrobot Mk IV User Manual page 19

Chapter | Operating Instructions
The light in the climate chamber can be switched off as well ("Light is On" changes into "Light
is Off" upon activation).
A chronometer is added to record experimental times. Once a specific time is set, the
chronometer starts to count down displaying a counterclockwise movement of the clock.
In the Options screen, additional vitrification parameters can be set. For example – under the
section Process Parameters - the relaxation time before the blotting ('wait time') and the
intermediate time between blotting and plunge freezing (drain time).
Parameters that affect the blotting process are the number of blottings (Blot Total), the time of
each individual blot ('blot time') and the force of the blotpads onto the grid ('blot Force'). The
latter determines the force at which the excess of fluid is removed from the grid.
The last possibility is to skip application of sample.
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PN 103261 | Revision D | 23-Oct-2019
User Manual
Page 19