Thermo Scientific Vitrobot Mk IV User Manual page 20

Chapter | Operating Instructions
One of the features is the option to do repetitive sample application onto the grid and
subsequent blotting prior to plunge freezing. To activate this function press Add and define
the application parameters of the first substance that is to be applied ont o the grid. In the
Processes section of the screen, the parameters are displayed. By again pressing ADD, the
application parameters for the second substance can be defined – as displayed in Processes.
Up to 20 application cycles can be added in this way (1,2,3,4....20).
The Console Screen also gives the opportunity to control the mouse sequence of the
complete vitrification process ('Place New Grid', 'Start Process', etc) or to Exit the interface.
After 16 sequential blottings, the "reset blot paper" button becomes "Red" pointing out that the
blot papers need to be replaced.
The memobox on the left side of the interface functions as an event logger. All major actions
and warnings are displayed there.
By pressing Delete selected application parameters can be removed from the sequence list.
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PN 103261 | Revision D | 23-Oct-2019
User Manual
Page 20