Thermo Scientific Vitrobot Mk IV User Manual page 25

Chapter | Operating Instructions
When the central cup is at liquid nitrogen temperature, ethane or propane can be condensed
in this cup (check for remaining liquid nitrogen in the center part, wait for complete
evaporation). While condensing the ethane or propane keep monitoring the peripheral ring;
refilling this reservoir with liquid nitrogen might be necessary. If the ethane or propane does
not condense, the metal parts are not at liquid nitrogen temperature yet. To speed up the
condensation and temperature decrease of liquid ethane/propane, the metal spindle can be
positioned on top of the central cup (with its "feet" into the pre-cooled peripheral ring). The
central cup should be filled up to the brim with liquid ethane or propane for optimal
vitrification. Indicative for the optimum freezing temperature of the ethane may be a white
solidified halo of ice on the inner side of the central cup. Immediately after the appearance of
this halo of ice, the metal spindle must be removed. See also the instruction video for more
visualized details.
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PN 103261 | Revision D | 23-Oct-2019
User Manual
Page 25