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R
PixelPro 3.2 Instruction Manual
Auto Bright: Uses algorithms to automatically separate bright objects from a dark
background.
Auto Dark: Uses algorithms to automatically separate dark objects from a bright
background.
Manual: Manual separation is based on the histogram distribution of the image, which
can be adjusted with the two vertical lines on the left and right in the histogram, or
adjusted directly by inputting values in the upper and lower limit edit boxes of the
histogram.
Dilate: Expands the borders of bright cells and shrinks the borders of dark cells in the
image.
Erode: Expands the borders of dark cells and shrinks the borders of bright cells in the
image.
Open: Changes the shape of cells in the image. For example, if the image is of a bright
cell on a dark background, this function will smooth the cell boundary, separate
connected cells, and remove small black holes in the cell.
Close: Change the shape of cells in the image. For example, if the image is of a bright cell
on a dark background, this function will fill in gaps in the cell, and stretch and highlight
cells near it.
Fill holes: Fill holes in image cells.
Restart Counting: Returns to the Start Counting interface.
Back: Returns to the previous operation process.
Next: Go to next step.
Contour: Uses contour lines to represent separated cells.
Area: Uses padding to represent separated cells.
Auto Cut: Draws boundaries directly onto the actual contour of separated cells.
Manual: Allows manual selection of multiple points on the interface, and separates cells
according to the selected points.
No Cut: Do not separate cells.
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