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PixelPro 3.2 Instruction Manual
3) To standardize the background for flat field correction, it is recommended to use
plastic, ceramic or professional white balance paper as the standard samples.
4) Flat Field Calibration requires that the background it uses as reference to be blank
and illumination on it to be uniform. Calibration should be run again if the camera
lens or the microscope objective is changed.
Default: Settings will restore to default values when [Default] is clicked. Color tune
default is 180, contrast default is 33, saturation default is 64, image enhancement save
defaults to unchecked, detail enhancement default is 1, and noise reduction default is 1.
3.8

Histogram

The user may perform adjustments to color levels to obtain more realistic images for
observation and analysis.
Color level adjustment means redefining the R/G/B color levels in each channel and then
re-allocating pixel values. Adjust color levels (gradations) to increase or decrease the
range of the selected area in the image, tuning it to brighter or darker values. The user
can also adjust the individual components of the three RGB channels separately to
change the image color via the corresponding channel(s).
Manual Color Level: The user can manually adjust the image's dark tone (left gradation),
gamma. and highlight brightness level (right color gradation) on the histogram to
regulate the image's tones, such as contrast, shading and image layers, to obtain the
correct balance for the whole image.
Auto Color Level: Check [Auto Min] and [Auto Max] to automatically set the brightest
and darkest pixels in each channel to white and black, and proportionally re-allocate
pixel values between them.
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