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Labomed Pixel Pro 3.2 User Manual

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Pixel Pro 3.2
User Manual
To ensure proper use of this instrument as well as to avoid injury while operating instrument, understanding this manual completely
before use is highly recommended.
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  • Page 1 Pixel Pro 3.2 User Manual To ensure proper use of this instrument as well as to avoid injury while operating instrument, understanding this manual completely before use is highly recommended.

  • Page 2: Table Of Contents

    Content PixelPro 3.2 Instruction Manual General Introduction ......................4 1. Starting Interface ....................... 5 2. Windows ........................6 3. Capture ........................11 Capture ......................11 Resolution ..................... 11 Binning ......................12 Exposure Control ..................12 White Balance ....................13 Bit Depth (only for Monochrome Cooling camera) ........14 Image Adjust ....................
  • Page 3 PixelPro 3.2 Instruction Manual Image Dye ..................... 32 Fluorescence....................33 Advanced Computational Imaging ............... 35 Binarization ....................36 Histogram ..................... 36 Smooth ......................37 Filter/Extract/Inverse Color ................38 Deconvolution ....................38 4.10 Automatic Counting ..................39 4.11 Automatic Counting Property ..............42 5.
  • Page 4 PixelPro 3.2 Instruction Manual Preferences ....................62 Help ......................63 About ......................63 Customer Service ....................64...

  • Page 5: General Introduction

    PixelPro 3.2 Instruction Manual General Introduction PixelPro is a powerful software package integrating controls for micro-imaging cameras, imaging calculation, image processing, and a measuring and counting work flow, allowing the user to enjoy an improved operating experience. PixelPro can drive and control cameras installed on microscopes from different manufacturers, helping to bring the user the best performance for micro-imaging tasks.

  • Page 6: Starting Interface

    PixelPro 3.2 Instruction Manual 1. Starting Interface When installing and starting up the software for the first time, the biological or industrial application option box will pop up. The software will automatically optimize parameter settings based on user choice. While using the software, the application option can also be switched through [Information] - [Options] - [Microscope] on the right upper part of the menu bar.

  • Page 7: Windows

    PixelPro 3.2 Instruction Manual 2. Windows The program home screen consists of five parts - status bar, control bar, preview window, data bar, picture bar: ① Status Bar There are seven main modules in the status bar: Capture / Image / Measure / Report / Camera List / Display / Config / Info.
  • Page 8 PixelPro 3.2 Instruction Manual Multi-camera connection and hot-plug functions are supported on Windows systems only. Using USB3.0 ports for hot swap is strongly recommended, and do not connect or disconnect cameras while the camera list is being refreshed. In the camera list, all recognized camera models are displayed. Click a camera name to switch to that camera.
  • Page 9 PixelPro 3.2 Instruction Manual will appear. The software supports four image formats for saving or saving as: [JPG] [TIF] [PNG] [DICOM]. ④ Data Bar Shows measurement and statistics tables. The measurement table supports exporting custom templates. For specific operations, please refer to the report chapter. ⑤...
  • Page 10 PixelPro 3.2 Instruction Manual 2) Right click on an image. The context menu of possible operations will appear as shown below. Available commands are [Select All (A)], [Cancel All], [Open], [New Folder (F)], [Copy (C)], [Paste (V)], [Delete (D)] and [Rename (M)]; the user can use ctrl+c shortcut keys to copy images, ctrl+v to paste it to the current folder.
  • Page 11 PixelPro 3.2 Instruction Manual There are two contrast mode: dynamic contrast and static contrast.  Dynamic contrast: Contrast between a preview image and a saved picture. In preview status, right-click the picture bar to pop up the operation menu, then select [Contrast].

  • Page 12: Capture

    PixelPro 3.2 Instruction Manual Capture Takes pictures and automatically saves the pictures taken. Record videos F11 Press once to record. Press again to stop recording. Stops displaying thumbnails on the right side. A prompt Close all dialog appears asking whether to close all pictures. Click [Yes] to confirm.

  • Page 13: Binning

    PixelPro 3.2 Instruction Manual Binning Binning: Binning mode can improve image sensitivity. Increasing the value increases the sensitivity. Note: This function performs sum binning based on the original data; the amount of data does not change, so the frame rate does not change. Exposure Control Set the exposure time of the camera.

  • Page 14: White Balance

    PixelPro 3.2 Instruction Manual The default setting for exposure is [Auto Exposure ]. White Balance The user can adjust white balance parameters according to the current light source to obtain better image effects . White Balance: By adjusting the ratio of the three individual components of red, green and blue, the camera is able to reflect the true image color under various illumination conditions.

  • Page 15: Bit Depth (Only For Monochrome Cooling Camera)

    PixelPro 3.2 Instruction Manual settings. The default setting for white balance is [Auto-white balance]. Bit Depth (only for Monochrome Cooling camera) The preview image displayed by default is an 8-bit data image. PixelPro allows the user to select 8 or 16-bit data as required by the user’s specific needs . Notes: ...
  • Page 16 PixelPro 3.2 Instruction Manual Contrast: Higher contrast yields a clearer picture with brighter colors. Setting ranges from 0 to 63. Sharpness: Improves clarity of picture edges. DPC: Reduces image bad points. Ranges from 0 to 3. 3 is default for FL-20. Black level: Adjusts gray value of dark field backgrounds.

  • Page 17: Histogram

    PixelPro 3.2 Instruction Manual 3) To standardize the background for flat field correction, it is recommended to use plastic, ceramic or professional white balance paper as the standard samples. 4) Flat Field Calibration requires that the background it uses as reference to be blank and illumination on it to be uniform.

  • Page 18: Temperature Control (Only For Cooling Camera)

    PixelPro 3.2 Instruction Manual Gamma: Adjusts the median of color levels. Setting ranges from 0.64 to 2.55. Histogram display: Histogram supports linear or logarithmic mode. Default: Click the [Default] button to restore the module's parameters to factory defaults. Color level adjusting defaults to manual, and the default gamma value is 2.10. Notes: 1) Calculating and displaying the histogram curve takes up some system resources, due to running data statistics in real time.

  • Page 19: File Save

    PixelPro 3.2 Instruction Manual 3.10 File Save PixelPro takes data from the real-time video data stream and records it in image format for later development and analysis. Click the button to capture a live preview image. Save Dialog Window: The file save window appears when the Capture button is clicked. Five file formats for saving pictures are supported: JPG, TIF, PNG, DICOM, and MPTIF.

  • Page 20: Roi

    PixelPro 3.2 Instruction Manual formats are selected simultaneously. 1) JPG: A lossy, compressed image format. Its image size is small, but the final quality of the image is low compared with the original captured image. 2) TIF: A lossless image format, which can save all the data transmitted from camera to saving device without losing data.

  • Page 21: Mask

    PixelPro 3.2 Instruction Manual Select ROI: Use a computer mouse. Check this box, then click and drag within the image preview to define the window area of the ROI. This window area displays the coordinate values and resolution of the current running task. Click on the [√] below the cursor to apply the ROI settings.

  • Page 22: Image Stitching (Live)

    PixelPro 3.2 Instruction Manual 3.13 Image Stitching (Live) The real-time image stitching function joins several individual images with overlapping features of parts of the specimen or sample, combining them into a single image showing the whole of the specimen. Stitching Speed: Two options: High Speed and High Quality. Default is High Speed. Background Color: The default background color of ignored areas on the stitched image is black, but can be customized.

  • Page 23: Edf (Live)

    PixelPro 3.2 Instruction Manual 3) If the navigation display frame is red, it means that the stitching area has been exceeded and stitching cannot be performed. The sample needs to be moved back to a location near the last stitching area. 4) Click [Stop Stitching] to end stitching.

  • Page 24: Realtime Dye

    PixelPro 3.2 Instruction Manual Quality: The higher the quality level of the new image requires, the longer it will take to merge its constituent images. Operating Procedure 1) Click the [Start EDF] button to start the extended depth of field operation. 2) Slowly turn the focus fine-tuning knob of the microscope to move the focus plane through the objective.

  • Page 25: Video Record

    PixelPro 3.2 Instruction Manual Apply: Applies the selected dye color to the image. Cancel: Stops applying any dyes added through custom mode. 3.16 Video Record Click on [Video Record] to save image data into video formats for playing back to observe the process of a sample’s live movements.

  • Page 26: Delay Capture

    PixelPro 3.2 Instruction Manual Save to RAM: Image data is temporarily saved in RAM; then, after image capturing is complete, all the image data is transferred to the hard drive. Select Save to RAM and enable RAM to save images. The maximum number of images that can be saved in RAM will be shown.

  • Page 27: Trigger (Only For Monochrome Cooling Camera)

    PixelPro 3.2 Instruction Manual Capture Frame: Begins capturing images according to the parameters set and saves each frame as an individual image. Click [Stop] to terminate the capturing task early. Capture as Video: Begins capturing images according to the parameters set and convert them into a video directly.

  • Page 28: Image Process (Only For Monochrome Cooling Camera)

    PixelPro 3.2 Instruction Manual There are several configuration modules: 1) Exposure: Timed: Exposure time is set by the program. Pulse width: Exposure time is set by input level width. 2) Edge: Rising edge: indicates that the trigger signal is valid for rising edge. Falling edge: indicates that the trigger signal is valid for falling edge.

  • Page 29: Save Settings

    PixelPro 3.2 Instruction Manual obtain a better imaging effect. Notes: 1) An appropriate number of accumulated frames should be set for integrated photographing, otherwise the image brightness will be too high and distortion will occur. 2) “Integration frame number” and “integration time length” cannot be enabled at the same time.

  • Page 30: Light Frequency

    PixelPro 3.2 Instruction Manual Parameter group files are cross-platform compatible, ensuring the user’s experiment conditions are as uniform as possible. Group Name: Gives the parameter group a user-defined name. The drop-down menu will display other parameter groups which have been saved or loaded. Save: Saves the current parameters into a named parameter group file.

  • Page 31: Other Settings

    PixelPro 3.2 Instruction Manual 3.22 Other Settings Negative: Inverts the current image colors . HDR: Check to achieve higher dynamic range and capture more image details for better image quality. 3.23 Auto Focus (only for Auto Focus camera) Continuous Focusing: Select focus area in preview screen. Camera will conduct continuous focusing on the selected area until it’s completely clear.

  • Page 32: Image Adjust

    PixelPro 3.2 Instruction Manual Click the screenshot button upper right corner of the preview window to crop the picture. Select the desired area in the preview image with the mouse, then double left click or double right-click to complete the screenshot. The screenshot will appear in the picture bar to the right.

  • Page 33: Image Dye

    PixelPro 3.2 Instruction Manual Brightness: The brightness of the image. Default value is 0. Adjustment ranges from – 255 to 255. Gamma: Adjusts the brightness of the image displayed on the monitor. Default value is 1.00. Adjustment ranges from 0.01 to 2.00. Contrast: The ratio between the darkest and brightest areas of the image.

  • Page 34: Fluorescence

    PixelPro 3.2 Instruction Manual Current: Shows the currently selected dye type. The program provides seven commonly used colors. Click to open the color selection palette shown below. Dye Type: Right-click to add custom colors to color groups future use. Add to New Dye: Adds the selected color on the palette as a new dye. Cancel: Cancels adding custom colors to dyes.
  • Page 35 PixelPro 3.2 Instruction Manual Operating Procedure: 1) Open the image which needs combined fluorescent channels in the preview window. 2) Check [Start Color Composite] to select a group of images with the same observation field as the one already open. A maximum of 4 images can be selected to combine together.

  • Page 36: Advanced Computational Imaging

    PixelPro 3.2 Instruction Manual potentially lead to pixel drift from one captured single-channel image to another, such that these captured single-channel images are not exactly aligned under the same observation field and area. The Drift Correction module allows the user to correct this pixel drift and ensure all single-channel images under the exact same observation field and area are properly aligned, resulting in perfect multi-channel combining.

  • Page 37: Binarization

    PixelPro 3.2 Instruction Manual 5) Click the [Apply as a New Image] button. The new combined image will be added to the image gallery and shown in the preview window. The combining process is then complete. Binarization PixelPro provides image binarization, which sets the value of the pixels on a grayscale image to either 0 or 255 based on a user-defined threshold.

  • Page 38: Smooth

    PixelPro 3.2 Instruction Manual Color Scale Adjustment: Redefines R/G/B in each channel, and then proportionally redistributes pixel values between them. Adjusting the color scale of a picture can enhance highlighted areas and brighten the whole picture, as well as darken too-bright pictures;...

  • Page 39: Filter/Extract/Inverse Color

    PixelPro 3.2 Instruction Manual Apply: When parameters are adjusted to desired values, click [Apply] to make the parameter settings take effect on the original image and generate a new image. The new image can be saved to other paths or documents. Cancel: Click the [Cancel] button to dismiss the module dialog.

  • Page 40: Automatic Counting

    PixelPro 3.2 Instruction Manual Note: This function is not supported on the Mac version. 4.10 Automatic Counting The Automatic Counting setting interface is shown below. Start Counting: Click to enter the counting process. All: The counting area is the full image. Rectangle: Left-click to diagonally opposite corners of a rectangular area.
  • Page 41 PixelPro 3.2 Instruction Manual Auto Bright: Uses algorithms to automatically separate bright objects from a dark background. Auto Dark: Uses algorithms to automatically separate dark objects from a bright background. Manual: Manual separation is based on the histogram distribution of the image, which can be adjusted with the two vertical lines on the left and right in the histogram, or adjusted directly by inputting values in the upper and lower limit edit boxes of the histogram.
  • Page 42 PixelPro 3.2 Instruction Manual Merge: Merges separate cells into one cell. Bound Process: When calculating the number of cells, cells with incomplete boundaries in the image will not be counted. Restart Counting: Returns to the Start Counting interface. Back: Return to the previous operation process. Next: Go to next step.

  • Page 43: Automatic Counting Property

    PixelPro 3.2 Instruction Manual draw any missed cells on the interface. Export Report: Exports statistical cell data to an Excel file. Restart Counting: Returns to the Start Counting interface. Back: Returns to the previous operation process. 4.11 Automatic Counting Property Font: Sets font and size.

  • Page 44: Measure Tool

    PixelPro 3.2 Instruction Manual Measure Tool Straight Line: Draws a line segment graphic with arrows at each endpoint. Hold [ctrl] to draw horizontal or vertical lines. H Shape Straight Line: Draws a line segment with a short perpendicular line at each endpoint.
  • Page 45 PixelPro 3.2 Instruction Manual click. Double left-click completes the drawing. Polyline: Left click adds new line segments to the existing polyline. Double left-click completes the drawing . Rectangle: Draws an orthographic rectangle. The length, width, perimeter and area of the rectangle can be measured. Polygon: Left-click multiple times to set the number of sides of a regular polygon.

  • Page 46: Calibration

    PixelPro 3.2 Instruction Manual Paintbrush: Draws a freehand curve on the preview screen. Arrow: Draws a pointer arrow with two clicks. Text: Adds a text note to the image. Select: Graphics or text notes drawn on the image can be selected. When the cursor, moved to the vicinity of graphics or text, changes from , the element can be dragged to a new position or deleted by pressing [Delete].
  • Page 47 PixelPro 3.2 Instruction Manual inch. Operating Procedure: 1) Click [Draw] and draw a straight line on the image. Hold [ctrl] to draw horizontal or vertical lines. 2) Enter the length and units represented by the number of pixels of the straight line. 3) Enter a name for the calibration scale.

  • Page 48: Measure Layer

    PixelPro 3.2 Instruction Manual Measure Layer Measure Layer: The user can create multiple layers on the image and make different measurements on different layers. This layer creating module meet the needs of a large number of image measuring and image processing applications. If some targets on the current image have already been measured, the layer creating function will automatically create a [Background] layer for those existing measurements and display the new measurements on [Layer 01].

  • Page 49: Metrics Flow

    PixelPro 3.2 Instruction Manual Rename: Renames the selected layer. Metrics Flow PixelPro provides a powerful metrics flow function for convenient and semi-automatic measurements in the pass rate detection of batches of industrial devices or parts. Operating Procedure: 1) Open a group of device or part images saved in the image gallery. 2) Select the image of the standardized sample (the “reference image”) to calibrate and set the tolerances for later measurements and detections .
  • Page 50 PixelPro 3.2 Instruction Manual template a name. [Save] completes recording and saves the measurement flow template. 6) Click [Start Applying A Metrics Flow], select the created template, then click [Run] to apply the template. Click [Delete] to delete the template if it is no longer needed. 7) Select the first image to be detected and compared to the template .

  • Page 51: Graphics Properties

    PixelPro 3.2 Instruction Manual 10) Click [Run] to select the next image to be detected. Repeat steps 7 through 9 above for each image to be compared against the reference image. 11) After all images to be detected have been processed, click [Stop Applying a Metrics Flow] to stop the measurement flow module.

  • Page 52: Manual Class Counting

    PixelPro 3.2 Instruction Manual color is blue. Click on the color swatch to select the desired color. No Background: True is checked by default to set the text background as transparent. Uncheck to enable the Background Color option below. Background Color: Sets the text background color for measurement graphics displayed on the image.

  • Page 53: Scale Property

    PixelPro 3.2 Instruction Manual Undo: Undoes the last action. Reset: Clears all classes and the counter table. Default: Resets parameters for all classes without clearing them. Start Class Counting: Select a category, then left click on the targets in the image to count. Count results will be automatically displayed on the data table.
  • Page 54 PixelPro 3.2 Instruction Manual Color: Sets the color for the current scale. Default color is red. Click the color swatch to open the color selection palette. Width: Sets the width value for the scale applied on the image. Default value is 5. Setting ranges from 1 to 5.

  • Page 55: Ruler Property

    PixelPro 3.2 Instruction Manual Ruler Property PixelPro allows the user to customize the crosshair properties of the current image. Show Ruler: Check this box to display crosshairs on the image. Default state is off. Unit interval: Sets interval distance for currently applied crosshairs. Ruler height: Sets height of currently applied crosshairs.

  • Page 56: Save Settings

    PixelPro 3.2 Instruction Manual Show Grid: Check to display a grid on the image. This setting is off by default. Type: Selects the style of grid to apply to the current image. Row/Column: If the line number grid type is selected, these two settings define the quantity of rows and columns applied to the image.

  • Page 57: Fluorescence Intensity

    PixelPro 3.2 Instruction Manual Load: Loads selected settings. Delete: Deletes selected settings. Export: Exports selected settings. Import: Imports settings file into drop-down menu. Reset All: Clears all user settings and reverts to factory settings. 5.11 Fluorescence Intensity Mosaic allows the user to measure the gray value of an image, and supports both linear and rectangular methods.

  • Page 58: Cursor Property

    PixelPro 3.2 Instruction Manual 5.12 Cursor Property The user can customize the properties of the measurement cursor. The setting interface is shown below. Width: Sets the thickness of the crosshair line segments. Default value is 2. Setting ranges from 1 to 5. Cross style: Sets the line style of the crosshairs.
  • Page 59 PixelPro 3.2 Instruction Manual Report Templates: Selects a template from the drop-down menu. The default PixelPro template is pre-selected. Add: Adds a custom template. Custom templates must be based on the software default template. The default template is the templates file in the software installation path. Use the # identifier to indicate content that needs to be displayed.

  • Page 60: Report

    PixelPro 3.2 Instruction Manual Report Report Templates: Sets the desired report template format. Project Name: Identifies the current project. This custom will show up on the working report. Sample Name: Identifies samples or parts. This custom name will show up on the working report.

  • Page 61: Display

    PixelPro 3.2 Instruction Manual This option is on by default. Measure Data: Hides or displays measurement data for the image included in the report. This option is on by default. Count Table: Hides or displays the count table for the image included in the report. This option is on by default.

  • Page 62: Config

    PixelPro 3.2 Instruction Manual 8. Config Capture/Image/Measure Software function modules and commands can be customized as shown below. Visible: Check a module to display it in the software interface. Unchecked modules will not be displayed in the interface. All modules are checked by default. The program remembers this setting between sessions.

  • Page 63: Jpeg

    PixelPro 3.2 Instruction Manual Jpeg PixelPro provides customizable Jpeg format size settings. When Jpeg is selected in the file saving module, the image size will be generated according to the following settings. Default: When the default is selected, the generated image keeps the current camera image resolution.

  • Page 64: Help

    PixelPro 3.2 Instruction Manual Microscope Option: Toggles between microscope applications after initial selection at program start-up. The program must be restarted before this setting takes effect. Help View the program operating instructions for reference by the user. About Click More information to view details about the camera, the current software version and operating system information.

  • Page 65: Customer Service

    PixelPro 3.2 Instruction Manual 10. Customer Service Warranty 1) When the product is under warranty, we will fulfill repairs for any damages corresponding with the warranty policies free of charge. 2) The warranty policies are limited to defects in manufacturing and materials. Any damage caused by the user, such as disassembly, liquid damage, or impact, or by natural disasters is not covered by the warranty.
  • Page 66 PixelPro 3.2 Instruction Manual PixelPro 3.2 Instruction Manual To save time, please have the following information ready: 1) Camera model and S/N (product serial number). 2) Software version number and computer system configuration information. 3) As many details as possible of the problem and images to help detail the problem.
  • Page 67 Fremont CA 94538 sales@laboamerica.com LABOMED and Pixel Pro™ are registered trademarks of Labo America, Inc. With a policy of continuous development, Labo America, Inc. reserves the right to change design and specifications without prior notice. ISO 9001 : 2008 © 2011 Labo America, Inc.

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