Install Liquid Addition Systems - NEW BRUNSWICK SCIENTIFIC BioFlo 110 Manual To Operations

56
AIR OUTLET BARB
(Connect to sterile inlet
filter on vessel headplate)
HANGING BRACKET
AIR INLET BARB
(Connect to clean air
source.)
3. Attach another piece of silicone tubing to the top air outlet hose barb (see Figure 34
above). Insert the other end of this tubing into the inlet sterile filter on the top of the
vessel headplate.
4. Verify that the exhaust port is connected to a filter. If you are using the optional
exhaust condenser, the filter should be attached to its outlet.
5. Read the airflow at the top flat edge of the float.
To increase airflow, turn the adjusting knob counterclockwise. To decrease airflow, turn
it clockwise.
Application
Fermentation
Cell Culture
NOTE:
Adjust the airlow according to your specific application. A commonly
suggested airflow for a typical culture is 1 VVM (vessel volume per minute) or
less for fermentation, and 0.05 VVM or less for cell culture; unnecessarily
high airflow can induce foaming.
4.9

Install Liquid Addition Systems

Addition ports are used to feed base, acid, nutrients and media into the vessel. To install
an addition system:
BioFlo 110 Modular Fermentor M1273-0054
Figure 34: Rotameter—Side View
Rotameter Maximum Flow Rate
1L
2.5 Lpm
0.5 Lpm
FRONT PANEL
GAUGE
AIRFLOW
ADJUSTMENT
KNOB
Vessel Size
3.0L 7.5L
5 Lpm 11.4 Lpm
1.2 Lpm 2.5 Lpm
14.0L
20 Lpm
5 Lpm
User's Guide