Confirm Ph Calibration; Install Liquid Addition Systems - NEW BRUNSWICK SCIENTIFIC BioFlo CelliGen 115 Manual To Operations

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4. Fit one side of the tubing loop into one of the spring-loaded clamps, pulling the
clamp open with your finger.
WARNING!
Be careful not to pinch your fingers in the pump head levers.
5. Then, as you rotate the pump's rotor by hand in a clockwise direction to clear the
channel, lay the tubing in the channel around the pump head.
6. Fit the other end of the tubing loop into the second spring-loaded clamp, making
sure the tubing fits tightly around the pumphead.
7. Press and hold the pump mode
100% setpoint and ensure that the pump operates smoothly.
See Section 11.1 for details on pump assignment and Section 11 for details on pump
set-up and operation.
9.3

Confirm pH Calibration

Autoclaving can alter the zero characteristics of pH probes, typically by 0.1-0.3 pH.
To check, and to compensate for any discrepancy, you will need an accurate external
pH meter.
1. Following sterilization, with the media at room temperature, note the pH value on
the BioFlo/CelliGen 115
2. Take a sample of media and measure the pH using the external meter.
3. If the two values disagree, return to the pH calibration screen (see Section 7.2) and
Set Zero to the value reported by the external meter. Do not change the Span or
you will invalidate the entire calibration.
The pH value will now agree with the external meter's reading.
9.4

Install Liquid Addition Systems

Figure 34 is a simple depiction of a typical addition system. Depending on the liquids
(base, acid, nutrients, media) to be added, your system may be slightly different.
1. Aseptically install (if applicable) a sterile (0.2µm) filter in one of the two
penetrations on the addition bottle cap.
2. Aseptically connect the tubing, securing it with a plastic tie, to the harvest tube in
the addition bottle. Clamp it off at the top.
3. If you have not already done so, thread the tubing through the selected feed pump.
BioFlo/CelliGen 115 M1369-0050
Prime
button or change the pump mode to
screen.
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