Inoculation & Addition Valve (Optional); Sterilization - NEW BRUNSWICK SCIENTIFIC BioFlo 4500 Manual To Operations

2 9 I
2 9 I
N O
N O
19 mm re-sterilizable inoculation and addition valves with quick-connect fittings are
optional. The two-position poppet addition valve is designed to attach to the vessel via a
retaining ring that holds the valve onto a welded fitting. A steam inlet line delivers
pressurized steam to the interior and exterior of the valve before and after each use.
Liquid is transferred via a quick-connect fitting. A stainless steel steam port cap
maintains the sterility of the fitting. Outlet steam is fed to a condensate trap. The valve
is designed to eliminate blind pockets and crevices. The two-position valve is spring-
loaded so that the entrance port opens a steam path though a hollow tube. When the
valve is in the forward position, the tube is extended into the vessel to allow sterile liquid
to be introduced into the vessel.
The prerequisites for sterile inoculum and addition transfer are:
• Sterile culture media in the culture vessel has cooled down to the operating
temperature.
• Adjustment of suitable airflow rate and set-up of sterile inlet and exhaust conditions.
• Adjusted agitation speed.
• Adjusted pH of the culture media.
• Suitable seed culture conditions.
29.1

Sterilization

To make an addition aseptically, the valve must be sterilized prior to use. It is a
good habit to re-sterilize the valve immediately after each use so that it is ready
for any subsequent addition.
To sterilize the valve (with reference to Figure 37 on the following page):
1. Ensure that the stainless steel cap is in place.
2. Open the
STEAM INLET VALVE
3. Allow steam to pass through the valve for about 15 minutes.
4. Close the
STEAM INLET VALVE
5. Allow the valve to cool thoroughly before use.
New Brunswick Scientific Co., Inc.
C U L A T I O N
C U L A T I O N
on the 19 mm port.
.
& A
& A
D D I T I O
D D I T I O
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O
O
179
V
V
N A L V E
N A L V E
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P T I O N A L
P T I O N A L
User's Guide