NEW BRUNSWICK SCIENTIFIC BioFlo 110 Manuals

NEW BRUNSWICK SCIENTIFIC BioFlo 110 Manuals

Manuals and User Guides for NEW BRUNSWICK SCIENTIFIC BioFlo 110. We have 1 NEW BRUNSWICK SCIENTIFIC BioFlo 110 manual available for free PDF download: Manual To Operations

NEW BRUNSWICK SCIENTIFIC BioFlo 110 Manual To Operations

NEW BRUNSWICK SCIENTIFIC BioFlo 110 Manual To Operations (182 pages)

Brand: NEW BRUNSWICK SCIENTIFIC | Category: Laboratory Equipment | Size: 5.86 MB

Table of Contents

Table of Contents

10
- Manual Conventions
  6
  - Table of Contents
    10
- Miscellaneous
  12
- Setting the Pumps
  12
- Design Concept
  14
- Process Control
  14
- Overview
  14
- Computer-Ready
  15
- Enhancing Your Fermentor
  15
- Inspection & Unpacking
  16
- Inspection of Boxes
  16
- Packing List Verification
  16
- Preparing the Location
  17
- Physical Location
  17
- Environment
  17
- Utilities
  17
- Primary Control Unit (PCU)
  18
- Installation, Assembly & Set-Up
  18
- Introduction to Control Module Rear Panels
  18
- Power Controller
  19
- Do2/Ph Controller
  20
- Level Controller
  21
- Gas MIX Controller
  21
- Pump Module
  22
- Other Optional Modules
  23
- Stacking Modules
  23
- Interconnecting the Modules
  25
- Important Warnings
  27
- Vessel Assembly: Non-Jacketed
  29
- Headplate
  31
- Install Heat Blanket
  33
- Install Vessel in Vessel Stand
  34
- Install Baffle (14.0L Fermentation Vessels ONLY)
  34
- Install Impeller(S)
  35
- Install Cooling Coil
  36
- Install Sparger (3.0L, 7.5L & 14.0L Vessels)
  36
- Install Harvest Tube
  37
- Install Sampler Tube
  37
- Install Thermowell
  37
- Install Foam Probe
  37
- Install Foam Exhaust Tube
  38
- Install Level Probe(S)
  38
- Install Addition Tube(S)
  38
- Install Ph Probe
  38
- Install Do2 Probe
  40
- Install Exhaust Condenser
  41
- Install Sampler
  43
- Sampler Tube
  44
- Install Foam Trap
  45
- Plug Unused Ports
  46
- Install 1.3L, 3.0L or 7.5L Fermentation Vessel Baffle
  47
- Install Headplate
  47
- Vessel Assembly: Water-Jacketed
  48
- Headplate
  50
- Install Headplate Clamping Ring
  53
- Install Vessel on Base Plate
  53
- Install Baffle (14.0L Fermentation Vessels ONLY)
  54
- Install Impeller(S)
  54
- Install Sparger
  55
- Install Foam Probe
  56
- Install Harvest Tube
  56
- Install Thermowell
  56
- Install Sampler Tube
  56
- Install Foam Exhaust Tube
  57
- Install Level Probe(S)
  57
- Install Addition Tube(S)
  57
- Install Ph Probe
  57
- Install Dissolved Oxygen Probe
  59
- Install Exhaust Condenser
  60
- Install Sampler
  63
- Install Foam Trap
  65
- Install Antifoam System
  66
- Plug Unused Ports
  66
- Install 1.3L, 3.0L or 7.5L Fermentation Vessel Baffle
  66
- Install the Agitation Motor
  67
- Install Headplate
  67
- Install the Rotameter
  68
- Install Liquid Addition Systems
  69
- Making Connections
  70
- Power
  70
- Water
  70
- Probes and Sensors
  71
- Pumps
  74
- Air and Optional Gases
  75
- Pump Flow Rates
  75
- Install Cable Guides
  76
- Voltage Selection and Fusing
  77
- Calibrating the Ph Probe
  78
- Assign Vessel Address
  82
- Select Pcu Display Language
  82
- Before First Use
  82
- For Pc Supervision
  83
- Connect to Supervisory System
  83
- Select Communications Protocol
  84
- Set Base Addresses
  85
- Set Chart Recorder for Data Logging
  85
- Sterilization & Reinstallation
  88
- Initial Preparation for Autoclaving
  89
- Filling the Water Jacket
  89
- Additional Preparation for Autoclaving
  89
- Autoclaving the Vessel
  90
- Sterilization Time and Temperature
  91
- Reinstalling the System
  92
- Polarizing & Calibrating the Do2 Probe
  93
- Confirming Ph Calibration
  93
- Powering the Jacket Water Heater
  95
- Operation
  97
- Primary Control Unit Display & Controls
  97
- Selecting the Application
  99
- Main Screen
  100
- Set the Temperature Loop
  102
- Control Mode
  102
- Setpoint
  104
- Graphic Display
  105
- Setting the Agitation Loop
  106
- Setting the Ph Loop
  106
- Setting a Deadband for Ph Control
  107
- Setting the Output Multiplier for Ph Control
  108
- Setting P & I Gains for Ph Control
  110
- Setting the Do2 Loop
  111
- Setting Do2 Control Cascades
  111
- Cascades for Fermentation Without Gas MIX Controller
  111
- Cascades for Fermentation with Gas MIX Controller
  113
- Cascades for Cell Culture Without Gas MIX Controller
  115
- Cascades for Cell Culture with Gas MIX Controller
  115
- Settingp & I Gains for D O2 Control
  118
- For Fermentation
  119
- For Cell Culture
  119
- 4-Gas Mode
  120
- Demand Mode
  120
- 100% Feed Mode
  120
- Pump Setpoint
  121
- Pump Setpoint and Multiplier
  121
- Pump Operation
  121
- Setting the Pumps
  121
- Control Mode
  122
- Setting Pumps
  122
- Setting the Pump Control Mode: Antifoam, Wet on & Wet off
  123
- Inoculation
  125
- Sterile Sampling
  125
- Automatic Foam Control
  125
- Harvesting
  126
- Maintenance
  127
- Cleaning the Modules
  127
- Ph Probe Maintenance and Storage
  127
- Do2 Probe Maintenance and Storage
  127
- Vessel Cleaning Methods
  128
- L Vessel Disassembly
  128
- Cleaning the Vessels
  128
- Port & Adapter O-Ring Replacement
  129
- Headplate O-Ring Replacement
  130
- Bearing Housing Cap Replacement
  130
- Replacing Pcu Fuses
  130
- Service
  131
- Troubleshooting
  131
- Replacement Parts & Accessories
  132
- Replacement Parts
  132
- Probes & Sensors
  134
- Replacement Vessels
  135
- Miscellaneous Parts & Optional Accessories
  135
- O-Ring Kit
  135
- 4-Pump Module
  136
- Module Kits
  136
- Appendix A: Specifications
  137
- Modules
  137
- Pump Flow Rates
  139
- Tubing
  140
- Vessels
  140
- Non-Jacketed Vessel Dimensions for Autoclaving (Vertical)
  140
- Non-Jacketed Vessel Dimensions for Autoclaving (Inclined)
  141
- Water-Jacketed Vessel Dimensions for Autoclaving (Vertical)
  142
- Non-Jacketed Vessel Dimensions for Autoclaving (Inclined)
  142
- Appendix B: Technical Interventions
  144
- Replacing Power Controller Fuses
  144
- Replacing Pcu Batteries
  145
- Voltage Selection
  146
- Converting from Single Fuse to Double Fuse
  147
- Changing Pcu Fusing
  147
- P&I Control Equations
  149
- Do2
  150
- Appendix C: Communications Protocols
  151
- Communications Settings
  151
- Afs/Modbus Pin Connections
  152
- AFS (Biocommand) Communications Protocol
  152
- General Specifications
  152
- Protocol
  153
- Requests & Response Headers
  153
- Settings for
  153
- Modbus Communications Protocol
  154
- Request Format
  154
- Message Formats
  154
- Change Commands
  154
- Register Map
  155
- Appendix D: Thermal Mass Flow Controller
  156
- Installation
  156
- Loading Software Update
  158
- Operation
  159
- Fermentation Mode Setup
  160
- Fermentation Mode Cascades
  162
- Sparger
  162
- Cell Culture Mode Setup
  163
- Cell Culture Mode Cascades
  164
- Cleaning
  165
- Specifications
  166
- Troubleshooting
  166
- Module Schematics
  167
- Appendix E: Drawings
  167
- Ph Probe
  175
- List of Drawings
  175
- Thermal Mass Flow Controller
  176
- Appendix F: Ports & Adapters
  177
- Bearing Housing Cap
  178
- Batteries
  178
- Angled Autoclave Rack
  178
- Addition/Harvest Kit
  178
- Accessories
  178
- Setting P & I Gains
  178
- Control Equation for Ph
  178
- Air Filter/Regulator Kit
  178
- Index
  178
- Headplate Port Fittings
  179
- Level Controller
  179
- Impellers
  179
- Pump Module
  179
- Fuses
  179
- Foam Trap Kit
  179
- Exhaust Condenser
  179
- Calibrating the
  179
New Brunswick Scientific User's Guide

180
- Option Part Numbers
  181
- Specifications
  181
- Spin Filter
  181
- Symbol For, V
  181
- Tubing
  181
- Vessel Assemblies
  181
- Vessels
  181
- Water Filter/Regulator Kit
  181

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