Prime The Fluid System; Install Enzyme Membranes - Xylem YSI 2500 User Manual

1. Unscrew and remove the lid from the empty calibrator bottle.
IMPORTANT: make every effort to avoid contamination of the lid and level sensor assembly.
2. Mark the date of installation on the label of the new bottle of YSI calibrator solution (the working life is 30 days).
3. Place the new bottle of YSI 2747 or 2776 calibrator in the bottle rack. Verify that the Calibrator setting in the
Configure screen matches the model number of the bottle you install.
4. Screw the lid and level sensor assembly onto it.
4.7

Prime the Fluid System

Please note that it may take from several minutes to more than an hour to initially stabilize the probes when setting up for
the first time (or after the power has been off for more than a few minutes).
To prime the fluid system:
1. From the Service screen, touch the [Module] tab.
2. Touch the button under B1 Pump to turn the buffer pump on.
3. The instrument will prime the buffer solution.
4. Once buffer flows from the end of the sipper, touch the button under B1 Pump to stop the pump.
5. Touch the button under Cal 1A Pump to turn it on.
6. The instrument will prime the calibrator solution.
7. Once calibrator flows from the calibrator well in the module, touch the button under Cal 1A Pump to stop the
pump.
Prime the calibrator bottle daily before calibrating or sampling to remove air bubbles from the tubing.
4.8

Install Enzyme Membranes

Each biosensor probe installed in your instrument is fitted with a protective "shipping membrane" which must be removed
and replaced with a new membrane. Make sure you install the correct membrane for each chemistry you are
measuring.
Enzyme membranes are color-coded, red for glucose and gray for lactate. It is important that you install the specific
membrane as indicated on each probe (A or B).
.
4-8