Sample Preparation; Run Batch; Create Batches - Xylem YSI 2500 User Manual

23. Listed in table 6-1 below are the recommended FCN limits.
a. Values less or equal to FCN limits indicate integral membranes
b. Values greater than FCN limits indicate membrane structural failure
c. If readings are high, recalibrate and repeat all the steps above to confirm.
d. If the reading is still out of tolerance, refer to Section 10 Troubleshooting.
Chemistry
Glucose
L-Lactate
24. See the list of acceptable values in table 6-2 below to interpret linearity readings.
a. Values that are ± 5% of specified tolerance limits indicate good membranes.
b. Values that are out of tolerance indicate an aging enzyme membrane.
c. If readings are out of tolerance limits, recalibrate and repeat all the steps above to confirm.
d. If the reading is still out of tolerance, refer to Section 10 Troubleshooting.
Chemistry
Glucose
L-Lactate
5.2

Sample Preparation

A variety of sample types can be analyzed with the 2500 Analyzer. Generally, the only sample preparation that may be
required is dilution of the sample to bring the substrate concentration within the linear range of the instrument (see Section
7.2 Measurement Parameter Information for the working range of each chemistry).
Neither color nor turbidity interferes with measurements.
Small particles do not affect the reaction in the sample module that houses the probes, but samples with particles large
enough to clog the sipper should be avoided.
5.3

Run Batch

5.3.1 Create Batches

1. From the Run Batch tab of the Run screen, select a sample rack/plate and create batches for your samples.
1
If you are using units other than g/L for the FCN test, refer to
Appendix B – Concentration Unit Conversion for conversion values.
Membrane Calibration Standard
2365 2776 or 2747
2329 2776 or 2747
Calibration Std Linearity Std
2776 (2.50 g/L) 1531 (9.00 g/L)
2776 (0.50 g/L) 1530 (2.67 g/L)
Table 6-1
Acceptable Range (g/L)
8.55 to 9.45
2.54 to 2.80
Table 6-2
5-5
1
FCN Limit
0.05 g/L
0.03 g/L