L-Lactate - Xylem YSI 2500 User Manual

7.2.2 L-Lactate

This is a direct reading of L-Lactate (L-Lactic Acid) in solution at the enzyme sensor. The enzyme L-Lactate Oxidase is
immobilized in the enzyme membrane.
L-Lactate + O
2 L-Lac Oxidase
System Buffer
Calibrator Std
Linearity Std
Membrane
Membrane
Color
Detection 0.05–2.70 g/L
Range
Calibration
Point
Linearity Check
Point
Sample Size
End Point
Precision 2% or 0.03 g/L,
(CV,n=10)
±2% or 0.03 g/L,
Linearity
(0.05 to Cal Point)
(Above Cal Point to
Typical Working
Life
Note: See Appendix B – Concentration
Unit Conversion if concentration unit
conversion is required.
H O + Pyruvate
2 2
Special Considerations:
YSI 2357
YSI 2776
YSI 1530
YSI 2329
Gray
0.5 g/L
2.67 g/L
25 µL
30 sec
whichever is
greater
whichever is
greater
±5%
Range Max)
14 days
•
If sample dilution is required, use reagent water.
•
D-Lactate is not a substrate for L-Lactate Oxidase.
Therefore, the 2500 Analyzer cannot directly measure
D-Lactate. If you have a known racemic mixture of
lactates, the L-Lactate value multiplied by 2 should give
you the total lactate value.
7-3