Check The Buffer Bottle; Check For Leaks; Clean Up Spills; Daily Operational Checks - Xylem YSI 2500 User Manual

8.2.3 Check the Buffer Bottle

Replace the buffer if the bottle is low or the buffer has been in the instrument longer than 1 week. Clean the buffer bottle
and cap with an authorized cleaning solution (see Section 8.1), then rinse well with authorized rinsing agent (see Section
8.1) before installing fresh buffer. You may find it convenient to make more than one liter of buffer at a time, in order to
have it on hand to replenish the buffer bottle. Prepare the buffer in a clean bottle with cap and store any excess at room
temperature.
After a buffer change, prime the buffer system (see Section 8.1). Buffer should be exiting the sipper inside the sample
module and overflowing to waste. You may need to initiate a second or third run of the buffer pump to complete the
priming process.

8.2.4 Check for Leaks

Examine the instrument for leaks. These are caused either by loose connections or worn tubing.

8.2.5 Clean up Spills

Spills should be cleaned up promptly to prevent biohazard build-up and corrosion. Clean any spills of biological material
from the sample area.

8.2.6 Daily Operational Checks

To verify proper instrument performance, perform the daily operational checks described in Section 5.1.1 Enzyme
Membrane Integrity Test and 5.1.2 Linearity Test.
8.3

Monthly Maintenance

Perform these procedures at least once a month to minimize the possibility of contamination. Depending on application
and use, more frequent cleaning may be required. The most convenient time to perform this maintenance is before
installation of a new bottle of calibration standard or buffer.

8.3.1 Calibration Pumping System Maintenance

Prepare about 100 mL of one of the authorized cleaning solutions (see Section 8.1) and place this solution in a clean
calibrator bottle. Install the bottle in the calibrator bottle position(s) you use.
From the Service screen, Module tab, touch [Off] to turn on the pump for the calibrator bottle position to flush the cleaning
solution through the pump tubing and calibrator well and to the waste bottle. After 3 minutes, touch [On] to turn the pump
off. Wait 7 minutes.
Remove and discard the authorized cleaning solution, then rinse the bottle thoroughly with authorized rinsing agent (see
Section 8.1). Next, add authorized rinsing agent to the bottle and reinstall the Cal bottle inside the bottle tray.
From the Service screen, Module tab, touch [Off] to prime the calibrator for 3 to 5 minutes to rinse the tubing and cal well.
After 3 to 5 minutes, touch [On] to turn the pump off.
Remove the Cal Cap Assembly. From the Service screen, Module tab, touch [Off] to flush the line with air. After 1 minute,
touch [On] to turn the pump off. Wipe the cal cap and steel tubes with a lint-free tissue.
Install a new bottle of calibration standard and mark the installation date on the bottle.
From the Service screen, Module tab, touch [Off] to prime the fresh calibrator through the tubing and cal well. After 2
minutes, touch [On] to turn the pump off.

8.3.2 Buffer Pumping System Maintenance

Prepare about 300 mL of one of the authorized cleaning solutions (see Section 8.1) and place this solution in a clean
buffer bottle. Install the bottle in the buffer bottle position(s) you use.
From the Service screen, Module tab, touch [Off] to turn on the pump for the buffer bottle position to flush the cleaning
solution through the pump tubing and to the waste bottle. After 3 minutes, touch [On] to turn the pump off. Wait 7 minutes.
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