Appendix A Optimizing The Lc Conditions; Flow Rates - Thermo Scientific Surveyor MSQ Plus Hardware Manual

Optimizing the LC Conditions

Flow Rates

Thermo Scientific
This appendix includes helpful information for optimizing your LC system and your
Cone Wash system.
Contents
• Flow Rates
• LC Solvents and Mobile Phase Additives
• Cone Wash System
• Flow Splitting
• PEEK Tubing
In general, the LC column employed determines the choice of flow rate. Each column has an
optimum flow rate. The guidelines in
Table 8. LC columns and flow rates
Column ID
4.6 mm
3.9 mm
2.1 mm
1.0 mm
Capillary
The different ionization modes require different flow rates and column IDs. The following
guidelines apply when using the Surveyor MSQ Plus MS detector:
• Electrospray can operate at all the flow rates described in
• APCI cannot operate at flow rates below 0.2 mL/min. Therefore, suitable column IDs are
2.1 mm, 3.9 mm and 4.6 mm.
Table 8 apply.
Flow Rate
1.0 mL/min
0.5 mL/min
0.2 mL/min
40-50 μL/min
<10 μL/min
A
Table 8.
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