Related Manuals for Thermo Scientific Sorvall Legend XF
Summary of Contents for Thermo Scientific Sorvall Legend XF
- Page 1 Thermo Scientific Sorvall Legend XF / XFR Centrifuge Instruction Manual 50156637-b • 03 / 2020 Visit us online to register your warranty: thermofisher.com/labwarranty...
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Page 2: Table Of Contents
Intended Use ..........................iii Accident Prevention ........................ iv Precautions ..........................iv Chapter 1 Introduction and Description ....................1-1 Characteristics of the Sorvall Legend XF / XFR ............1-2 Technical Data ......................1-3 Directives, Standards and Guidelines ................1-4 Functions and Features ......................1-4 Mains Supply ......................1-4 Rotor Selection ......................1-5... - Page 3 Service of Thermo Fisher Scientific ................6-5 Chapter 7 Trouble Shooting ........................7-1 Mechanical Emergency Door Release ..............7-2 Troubleshooting by User ..................7-3 When to contact Customer Service ................7-5 Chemical Compatibility Chart ................A-1 Sorvall Legend XF / XFR Thermo Scientific...
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Page 4: Preface
If any parts are missing, please contact your nearest Thermo Fisher Scientific representative. Intended Use • This centrifuge is designed to separate sample mixtures of different densities like chemicals, environmental samples and other non-human body samples. Sorvall Legend XF / XFR Thermo Scientific... -
Page 5: Accident Prevention
Accident Prevention Accident Prevention Prerequisite for the safe operation of the Sorvall Legend XF / XFR is a work environment in compliance with standards, directives and trade association safety regulations and proper instruction of the user. The safety regulations contain the following basic recommendations: •... - Page 6 • Highly corrosive substances which can cause material damage and impair the mechanical stability of the rotor, should only be centrifuged in corresponding protective tubes. IF A HAZARDOUS SITUATION OCCURS, TURN OFF THE POWER SUPPLY TO THE CENTRIFUGE AND LEAVE THE AREA IMMEDIATELY. Thermo Scientific Sorvall Legend XF / XFR...
- Page 7 WARNING means that injuries or material damage or contamination could occur. This symbol refers to biological hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe. vi Sorvall Legend XF / XFR Thermo Scientific...
- Page 8 Introduction and Description Contents “Characteristics of the Sorvall Legend XF / XFR” page 1-2 • “Technical Data” page 1-3 • “Directives, Standards and Guidelines” page 1-4 • “Functions and Features” page 1-5 • “Mains Supply” page 1-5 • “Rotor Selection”...
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Page 9: Chapter 1 Introduction And Description
Characteristics of the Sorvall Legend XF / XFR Characteristics of the Sorvall Legend XF / XFR The Sorvall Legend XF / XFR is designed to separate sample mixtures of different densities. Several rotors with a wide range of tubes can be used. -
Page 10: Technical Data
Introduction and Description Technical Data Technical Data The technical data of the Sorvall Legend XF / XFR is listed in the following table. Table 1-1. Technical Data Sorvall Legend XF / XFR Feature Value Environmental conditions -Use in interior spaces -Altitudes of up to 2,000 m above sea level -max. -
Page 11: Directives, Standards And Guidelines
Automatic lid closing and locking starting from an initial hold position Mains Supply The following table contains an overview of the electrical connection data for the Sorvall Legend XF / XFR. This data is to be taken into consider ation when selecting the mains connection socket. -
Page 12: Rotor Selection
Introduction and Description Rotor Selection Rotor Selection The Sorvall Legend XF / XFR is supplied without a rotor. Various rotors are available to choose from. TX-1000 7500 3017 with rectangular buckets 7500 3001 TX-750 7500 3180 with round buckets 7500 3608... - Page 13 Before use Contents “Before Setting up” page 2-2 • “Transporting the Centrifuge” page 2-4 • “Location” page 2-4 • “Aligning the Centrifuge” page 2-4 • “Mains Connection” page 2-5 • “Storage” page 2-6 • Thermo Scientific Sorvall Legend XF / XFR...
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Page 14: Chapter 2 Before Use
If the order is incomplete, please contact Thermo Fisher Scientific. Setting up 1. Place the pallet with the centrifuge so that you have at leased 1.5 m room in front of the pallet. 2. Release the rails from the pallet. 2-2 Sorvall Legend XF / XFR Thermo Scientific... - Page 15 4. Remove the protectors that locate the centrifuge on the pallet. Release the castors. Use several people in order to roll the centrifuge off the pallet. 5. Fix the castors once the centrifuge is in its final position. Thermo Scientific Sorvall Legend XF / XFR...
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Page 16: Transporting The Centrifuge
The supporting structure must be suitable for horizontal setup of the centrifuge. CAUTION If the centrifuge isn’t level, imbalances can occur and the centrifuge can be damaged. Do not place anything under the feet to level the centrifuge. 2-4 Sorvall Legend XF / XFR Thermo Scientific... -
Page 17: Mains Connection
3. Check whether the cable complies with the safety standards of your country. 4. Make sure that the voltage and frequency correspond to the figures on the rating plate. 5. Establish the connection to the power supply with the connecting cable. Thermo Scientific Sorvall Legend XF / XFR... -
Page 18: Storage
• Before storing the centrifuge and the accesories it must be cleanedand if necessary desinfected and decontaminated. • Store the centrifuge in a clean, dust-free location. • Be sure to place the centrifuge on its feet. • Avoid direct sunlight. • Fix the castors. 2-6 Sorvall Legend XF / XFR Thermo Scientific... -
Page 19: Control Panel
Control Panel Contents “Control Panel” page 3-2 • “Keys” page 3-2 • Thermo Scientific Sorvall Legend XF / XFR... -
Page 20: Keys
Acceleration Curves Use the Toggle key to change the display mode. (speed / RCF Changing the display value, sample / chamber temperature, run time counter from start or mode preset speed on) Thermo Scientific 3-2 Sorvall Legend XF / XFR... -
Page 21: Operation
“Entering Parameters” page 4-4 • “Centrifugation” page 4-10 • “Temperature Adaptation during Standstill” page 4-11 • “Short-term Centrifugation” page 4-11 • “Removing the Rotor” page 4-12 • “Aligning the Centrifuge” page 4-13 • Thermo Scientific Sorvall Legend XF / XFR... -
Page 22: Switch On Centrifuge
Note The lid should audibly click into place. Rotor Installation The approved rotors for the Sorvall Legend XF / XFR are listed in section “Rotor Selection” page 1-6. Use only the rotors and accessories from this list in the centrifuge. - Page 23 Check for any damage to the rotor: Damaged rotors must not be used. Keep the centrifuge spindle area of the rotor clear of objects. CAUTION Check that the rotor is properly locked on the centrifuge spindle before each use by pulling it at its handle. Sorvall Legend XF / XFR Thermo Scientific...
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Page 24: Entering Parameters
Entering Parameters Acceleration curve The Sorvall Legend XF / XFR offers you a total of 9 acceleration and 10 braking curves with which samples and gradients can be centrifuged. After the centrifuge is turned on, the last running profile selected is shown. - Page 25 This can be accounted for in the calculation above if required. Running time preselection 1. Press the TIME key in order to open the runtime selection menu. The display shows the message "Set Time". Enter the desired runtime in hh.mm Sorvall Legend XF / XFR Thermo Scientific...
- Page 26 Press the TOGGLE key to toggle between the two display modes. When AIR is lit, the temperature inside the rotor chamber will be controlled. This setting is recommended when using the centrifuges pretemp feature. 4-6 Sorvall Legend XF / XFR Thermo Scientific...
- Page 27 The digits show in sequential order. 3. Confirm your entry by pressing ENTER Your entry will be automatically confirmed if you do not press any key for 5 seconds. 4. The display shows: Thermo Scientific Sorvall Legend XF / XFR...
- Page 28 When you use adaptors, you will have to specify the radius. For appropriate values please refer to the instruction manual for the rotor. 1. Press ENTER to confirm your selection and open the radius input menu. 4-8 Sorvall Legend XF / XFR Thermo Scientific...
- Page 29 1. Select the program you wish to change. 2. Press the program memory key to load any of the programs 1-5. Press the DIRECTORY key in order to load any of the programs 1-99 by means of the numeric pad. Thermo Scientific Sorvall Legend XF / XFR...
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Page 30: Centrifugation
You cannot open the lid as long as the centrifuge is running. Imbalance indicator If a load is imbalanced, this will be indicated at speed higher than approx. 300 rpm by the message "Imbalanced load". The run will terminate. Thermo Scientific 4-10 Sorvall Legend XF / XFR... -
Page 31: Temperature Adaptation During Standstill
Short-term Centrifugation For short-term centrifuging, the Sorvall Legend XF / XFR has a PULSE- function. By holding down the PULSE key , spinning will start and continue until the key is let go. -
Page 32: Removing The Rotor
. Be sure not to place the lid onto this mandrel to prevent it from beingdamaged. Figure 4-2. Auto-Lock -lid for aerosol-tight rotors WARNING Mind the Auto-Lock mandrel inside the lid. Do not touch the mandrel. Thermo Scientific 4-12 Sorvall Legend XF / XFR... -
Page 33: Aligning The Centrifuge
Operation Aligning the Centrifuge Aligning the Centrifuge 1. To turn off the centrifuge put the mains switch to "0". Sorvall Legend XF / XFR 4-13 Thermo Scientific... -
Page 34: Chapter 5 System Menu
Lid passcode? Set lid passcode _ _ _ _ ENTER Program passcodes? ENTER Auto lid open? ENTER Software ID: 0547V01 0547V01 4641V05 ENTER 1 Software and NVRAM identification example display Cycle count: 2368 ENTER Sorvall Legend XF / XFR Thermo Scientific... -
Page 35: Description
Press ENTER key to confirm. Program passcodes Press 1 (yes) on the numeric pad if the centrifuge programs should be protected with a passcode. Otherwise press 0 (no). Press ENTER key to confirm. Sorvall Legend XF / XFR Thermo Scientific... - Page 36 Press ENTER key to confirm. Software ID Here you fine the current software version. Press ENTER key to confirm. Cycle count Here you fine the current numbers of cycles. Press ENTER key to confirm. Thermo Scientific Sorvall Legend XF / XFR...
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Page 37: Maintenance And Care
Maintenance and Care Contents “Cleaning Intervalls” page 6-2 • • “Cleaning” page 6-2 “Disinfection” page 6-3 • • “Decontamination” page 6-4 “Autoclaving” page 6-5 • • “Service of Thermo Fisher Scientific” page 6-5 Sorvall Legend XF / XFR Thermo Scientific... -
Page 38: Cleaning Intervalls
• After cleaning, treat the entire surface of aluminum parts with corrosion protection oil (7000 9824). Also treat the cavities with oil. • Store the aluminum parts at room temperature or in a cold-storage room with the cavities pointing down. Sorvall Legend XF / XFR Thermo Scientific... -
Page 39: Disinfection
CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. Observe the safety precautions and handling instructions for the cleaning agents used. Sorvall Legend XF / XFR Thermo Scientific... -
Page 40: Decontamination
For general radioactive decontamination use a solution of equal parts of 70% ethanol, 10% SDS and water. 1. Open the centrifuge. 2. Turn off the centrifuge. 3. Pull out the power supply plug. Sorvall Legend XF / XFR Thermo Scientific... -
Page 41: Autoclaving
The service technicians check the following: • the electrical equipment • the suitability of the set-up site • the lid lock and the safety system • the rotor • the fixation of the rotor and the drive shaft Sorvall Legend XF / XFR Thermo Scientific... - Page 42 This is only valid if the centrifuge has only been mainained by a Thermo Fisher Scientific service technician. 6- 6 Sorvall Legend XF / XFR Thermo Scientific...
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Page 43: Trouble Shooting
Trouble Shooting Contents “Mechanical Emergency Door Release” page 7-2 • “Troubleshooting by User” page 7-3 • “When to contact Customer Service” page 7-5 • Thermo Scientific Sorvall Legend XF / XFR... -
Page 44: Mechanical Emergency Door Release
Emergency Door Release Figure 7-1. Emergency Door Release Note You need to pull both levers in order to unlock both locks. 4. Push the cord back into the centrifuge and mount the plug. Sorvall Legend XF / XFR Thermo Scientific... -
Page 45: Troubleshooting By User
If an error message appears again, inform Customer Service. Motor overtemp. The centrifuge cannot be operated. Restart the centrifuge. If an error message appears again, inform Customer Service. The run does not start or the centrifuge runs down without being braked. Sorvall Legend XF / XFR Thermo Scientific... - Page 46 Time has expired for the lid lock drive while opening the lid. The run does not start or the centrifuge runs Restart the centrifuge. down without being braked. If an error message appears again, inform Customer Service. 7-4 Sorvall Legend XF / XFR Thermo Scientific...
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Page 47: When To Contact Customer Service
This information can be found on the back near the inlet for the power supply cable. To identify the software version, proceed as follows: 1. Hold down any of the keys and then switch on the centrifuge. You enter the system menu. 2. Press the START key Thermo Scientific Sorvall Legend XF / XFR... - Page 48 Trouble Shooting When to contact Customer Service 3. Press and hold the ENTER key , until the following message is displayed: Software ID: XXXXXXXX 4. Communicate the software version to the service technician. Sorvall Legend XF / XFR Thermo Scientific...
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Page 49: Chemical Compatibility Chart
S S S S S S M S S S Barium Salts S S U U S U M U S U U S U U U M U M U U U S U U S U S Benzene Sorvall Legend XF / XFR Thermo Scientific A- 1... - Page 50 S S S M S S S U S M U Ethyl Alcohol (95%) U U - S M - U U S U U U U U U U - U S U - Ethylene Dichloride A- 2 Sorvall Legend XF / XFR Thermo Scientific...
- Page 51 U U U U U U U - Aqua Regia S S S - S S S S S S S - S S S - S S S S S S Solution 555 (20%) Sorvall Legend XF / XFR Thermo Scientific...
- Page 52 Perchloric Acid (70%) U S U - S M M - S U M U U S U M S M S U U S U M M M S Phenol (5%) A-4 Sorvall Legend XF / XFR Thermo Scientific...
- Page 53 S S S S S S S S S S S S S S S S S S S Triton X-100 U S S S S - S S S M S S S S - S S S M S - Urea Thermo Scientific Sorvall Legend XF / XFR...
- Page 54 Chemical resistance data is included only as a guide to product use. No organized chemical resistance data exists for materials under the stress of centrifugation. When in doubt we recommend pretesting sample lots. A-6 Sorvall Legend XF / XFR Thermo Scientific...
- Page 55 Explanation of RCF Value ......4-5 Removing the Rotor ........4-12 Failure message ..........7-3 Rotor Installation ..........4-2 Features ............1-5 Rotor Selection ..........1-6 Running time preselection .......4-5 Imbalance indicator ........4-10 Intended Use ............v Safety features ..........1-2 Sorvall Legend XF / XFR Thermo Scientific...
- Page 56 Starting centrifuge program ......4-10 Stopping the centrifugation program ..... 4-11 Storage ............2-6 Switch on Centrifuge ........4-2 Technical Data ..........1-3 Temperature Adaptation .......4-11 Transporting the Centrifuge ......2-4 Trouble Shooting ..........7-1 I-2 Sorvall Legend XF / XFR Thermo Scientific...
- Page 57 Thermo Electron LED GmbH Zweigniederlassung Osterode Am Kalkberg, 37520 Osterode am Harz Deutschland thermofisher.com/centrifuge © 2020 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries unless otherwise indicated. Delrin, TEFLON, and Viton are registered trademarks of DuPont. Noryl is a registered trademark of SABIC. POLYCLEAR is a registered trademark of Hongye CO., Ltd.