Waters Taperslit Flow Cell - Waters 2487 Dual A Operator's Manual

The verification tests for the 2487 Detector require 5 minutes of lamp warmup time so the
lamp can stabilize.
When the 2487 Detector is run continuously, we recommend you perform wavelength
1
verification weekly by turning the detector off, then on again. See also
Wavelength

1.2.3 Waters TaperSlit Flow Cell

The Waters TaperSlit Flow Cell (U.S. Patent No. 5,883721, March 16, 1999) used in the
2487 Detector renders the detector baseline insensitive to changes in mobile phase
refractive index (RI). RI changes occur during gradient separations or result from
temperature or pump-induced pressure fluctuations.
To achieve RI immunity, a combination of a spherical mirror, a lens at the entrance of the
flow cell, and a taper to the internal bore of the flow cell prevents light rays from striking
the internal walls of the flow cell. An additional feature of the Waters TaperSlit Flow Cell
and the reason for its name is the shape of the flow cell entrance, which matches the
shape of the entrance slit. Compared to a conventional flow cell with a circular entrance,
the 2487 Detector achieves higher light throughput for a given spectral resolution with the
TaperSlit cell design.
30 2487 Detector Theory of Operation
Calibration.
Section 3.3.3,