Scanning Using A Flow Cell And A Syringe; Conserving Lamp Life - Waters 2489 Overview And Maintenance Manual
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Also See for 2489:
- Operator's manual (234 pages) ,
- Operator's manual (194 pages) ,
- Quick install manual (4 pages)
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Recommendations:
•
Ensure that you have enough liquid (3 mL) in the cuvette so that when it is
inserted into the holder, you can see liquid through the holder's aperture.
That is, the liquid must completely cover the aperture.
•
Because the cuvette holder is angled, use your thumb or forefinger to
ensure the cuvette is secure in the slot and does not slide forward. Ensure
that it does not become dislodged when you are replacing the cuvette
holder.
4.
Gently guide the cuvette holder back into the flow cell assembly until it bottoms
in the space.
5.
Close the door.
Rules:
•
To prevent invalid chromatographic results, remove the cuvette from the
detector and replace the empty holder after running cuvette scans.
•
To maintain optimum system performance, close the front door before
resuming normal operation of the detector.
6.
Insert a reference cuvette containing the mobile phase standard, and run a zero
scan.
7.
Replace the reference cuvette with a cuvette containing your analyte dissolved
in mobile phase solvent, and run a sample scan.
8.
Use the storage, review, subtract and review, and replay functions to analyze
the data obtained.
3.3.8 Scanning using a flow cell and a syringe
If you do not have a cuvette, you can scan using a manually filled flow cell.
Requirement:
cuvette holder.
To run a spectrum using a flow cell:
1.
With a syringe, fill the flow cell with the mobile phase or solvent in which your
sample is dissolved.
2.
Run a zero scan according to the procedure on
3.
With a syringe, fill the flow cell with the analyte, and then run a sample scan
according to the procedure on
Use the storage, review, subtract and review, and replay functions of the detector to
compare the scanned data.
3.3.9 Conserving lamp life
You can conserve the lamp without powering-off the detector by leaving the
instrument on and turning off only the deuterium lamp.
Tip:
If the detector is operating under remote control, you can program the controller
to turn the lamp off and on without using the front panel.
Before you scan using a flow cell, make sure no cuvette is in the
page
June 11, 2015, 715004752 Rev. A
Page 90
page
80.
80.
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