Maintenance Procedures; Taking Care Of The Flow Cell; Decontaminating The Fluid Path - Waters 2414 Overview And Maintenance Manual

6

Maintenance Procedures

Keep to a maintenance schedule and perform maintenance as described in this
chapter.
Detector maintenance involves cleaning the fluid path and replacing fuses.
Warning:
• do not open the detector's cover. The detector's components are not
• do not disconnect an electrical assembly while the detector is
Warning:
detector, always observe Good Laboratory Practices (GLP). Know the
physical and chemical properties of the solvents you use by referring to
their Material Safety Data Sheets.
Warning:
integrated circuit chips or other components that do not specifically
require manual adjustment.

6.1 Taking care of the flow cell

Notice:
exceed 1000 kPa (10.0 bar, 145 psi).
Tip:
To ensure a long life for flow cells and proper detector initialization, use
well-degassed eluents.
To clear bubbles from the flow cell, establish fluid flow through the detector flow cell
using degassed mobile phase at the flow rate anticipated for the subsequent analysis.
To prevent contamination of the flow cell, flush columns using at least 10 column
volumes of clean mobile phase before connecting them to the flow cell.

6.2 Decontaminating the fluid path

A contaminated fluid path can cause baseline noise, drift, inaccurate sample
refraction, and other operational problems. If you suspect the detector's fluid path is
contaminated, follow the procedure, below, to decontaminate it.
To avoid electrical shock,
user-serviceable.
powered-on.
When you handle solvents, change tubing, or operate the
To avoid damage caused by static electricity, do not touch
To avoid damaging the flow cell, do not allow the backpressure to
June 11, 2015, 715004754 Rev. A
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