Thermo Scientific Shake 'n Stack 6245 Manuals

Manuals and User Guides for Thermo Scientific Shake 'n Stack 6245. We have 1 Thermo Scientific Shake 'n Stack 6245 manual available for free PDF download: Operating And Maintenance Manual

Thermo Scientific Shake 'n Stack 6245 Operating And Maintenance Manual (92 pages)

Hybridisation Oven

Brand: Thermo Scientific | Category: Laboratory Equipment | Size: 11.94 MB

Table of Contents

6
- Introduction
  8
  - Oven Temperature Monitoring
    8
  - Safety Precautions
    9
- Unpacking and Installation
  10
  - Operation
    11
  - Set the Required Temperature
    11
- Set up the Shaking Platform
  14
  - Place Membranes in a Bottle
    16
  - Insert Membranes in a Bottle
    17
  - Hybridisation
    18
  - Pre-Hybridisation
    18
  - Washing
    19
  - Washing in Hybridisation Bottles
    19
  - Washing in a Sandwich Box
    20
  - Rotisseries
    22
  - Variable Axis Rotisseries
    22
  - 50Ml & 15Ml Tube Rotisseries
    23
- Cleaning and Decontamination
  24
  - Meshes
    24
  - Bottles & Caps
    25
  - Oven Interior/Shaking Platform
    25
  - Rotisserie
    25
- Use of Radioactive Probes
  26
  - Technical Specifications
    28
  - Additional Accessories
    30
  - Electrical Schematics
    38
- Appendix I Troubleshooting Guide for Nucleic Acid Hybridisations
  
  43
- Appendix II
  
  47
  - Hybidisation Bottle Safety
    47
  - Bottle Care
    47
H Gybridisationuide

52

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Thermo Scientific Shake 'n Stack 6245 Manuals

Thermo Scientific Shake 'n Stack 6245 Operating And Maintenance Manual (92 pages)

Table of Contents

Table of Contents

Appendix I Troubleshooting Guide for Nucleic Acid Hybridisations

Appendix II

H Gybridisationuide

Chapter 1: Introduction

Chapter 2: Nucleic Acid Transfer Procedures

Chapter 3: Hybridisation Procedures

Chapter 4: Nucleic Acid Probes

Chapter 5: Non-Radioactive System

Chapter 6: Hybridisation Using Oligonucleotide Probes

Chapter 7: Troubleshooting Guide for Nucleic Acid

Appendix I: Solutions for Nucleic Acid Blotting

Appendix II: Factors Affecting Stringency of Hybridisation Reactions

Appendix III: Hybridisation Bottle Safety

Appendix IV: References

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