How To Prevent And/Or Reduce The Algae Problem - Agilent Technologies 1200 Series Manual

4
Using the Quaternary Pump
Algae Growth in HPLC Systems
• Short lifetime of solvent filters (bottle head assembly). A blocked solvent
• Algae growth may also be the possible source for failures of the ball valves

How to Prevent and/or Reduce the Algae Problem

• Always use freshly prepared solvents, especially use demineralized water
• Never leave mobile phase in the instrument for several days without flow.
• Always discard "old" mobile phase.
• Use the amber solvent bottle (part number 9301-1450) supplied with the
• If possible add a few mg/l sodium azide or a few percent organic solvent to
54
normal wear of the piston seals do not cause the PTFE frits to block over
short-term usage. Please refer to the section
Frit or the Purge Valve"
filter in the bottle, especially when only partly blocked, is more difficult to
identify and may show up as gradient performance problems, intermittent
pressure fluctuations etc.
and other components in the flow path.
which was filtered through about 0.2 µm filters.
instrument for your aqueous mobile phase.
the aqueous mobile phase.
on page 85 in this manual.
1200 Series Quarternary Pump User Manual
"Exchanging the Purge Valve