Priming With The Pump - Agilent Technologies 1200 Series Manual

Priming with the Pump

When the pumping system has been turned off for a certain time (for example,
overnight) oxygen will rediffuse into the solvent channels between the vacuum
degasser and the pump. Solvents containing volatile ingredients will slightly
lose these, if left in the degasser without flow for a prolonged period of time.
Therefore priming of the vacuum degasser and the pumping system is
required before starting an application.
1 Open the purge valve of your pump (by turning it counterclockwise) and set
2 Flush the vacuum degasser and all tubes with at least 30 ml of solvent.
3 Set flow to required value of your application and close the purge valve.
4 Pump for approximately 10 minutes before starting your application.
5 Repeat step 1 to step 4 for other solvent channels, where needed.
Table 8
Activity
After an installation
When switching between
reverse phase and normal
phase (both times)
After an installation
To clean the system when
using buffers
After a solvent change
After the installation of normal
phase seals (P/N 0905-1420)
1200 Series Quarternary Pump User Manual
flow rate to 3-5 ml/min.
Choice of Priming Solvents for Different Purposes
Priming and Purging the System
Solvent
Isopropanol
Isopropanol
Ethanol or Methanol
Bidistilled water
Bidistilled water
Hexane + 5% Isopropanol
Installing the Pump
Comments
Best solvent to flush air out of
the system
Best solvent to flush air out of
the system
Alternative to Isopropanol
(second choice) if no
Isopropanol is available
Best solvent to re-dissolve
buffer cristals
Best solvent to re-dissolve
buffer cristals
Good wetting properties
3
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