Molecular Devices SkanStacker 300 User Manual page 52

Troubleshooting Tips
Problem
Cells are aspirated
off the bottom of the
wells.
Residual volume too
high or low.
SkanStacker 300 User Guide
Things to Try
Decrease vacuum pressure at pump by
increasing value. Optimize cell assay with
vacuum pressure of 350-450 mbar.
Cells are being disrupted during dispens-
ing. Lower air pressure by decreasing air
pressure value in software through Edit
Program.
Check Aspirate position in Edit Program
from the Setup Menu in the software. Re-
set height of aspiration probes. For low
residual volume, the aspirate probe must
be close to the bottom of the well.
Check Dispense position in Edit Program
from the Setup Menu in the software. Re-
set height of dispense probes.
Check vacuum pressure. Pressure setting
of 250 mbar is suggested for ELISA appli-
cations. Increasing the value decreases the
vacuum pressure, which slows the aspira-
tion rate. Use a slower aspiration rate when
working with cells.
Check Aspirate position in Edit Program
from the Setup Menu in the software. Re-
set height of aspiration probes. For low
residual volume, the aspirate probe must
be close to the bottom and side of the well.
If residual volume too low, check also for
clogged probes. Is volume low in certain
wells?
Troubleshooting
46