Advanced Maintenance; Dispense Test; Clogged Probes - Molecular Devices SkanStacker 300 User Manual

Maintenance

Advanced Maintenance

Dispense test

Step 1 Set up program for dispense test using the following parameters:
Aspirate 2 sec
Wash 350 µl
Aspirate 2 sec
Dispense 350 µl
END
Step 2 Prime system.
Step 3 Run program and inspect dispense levels; note any low volumes.
Step 4 Repeat 3 times.
Step 5 Inspect each well and note if any wells have lower volumes. If low
volume consistent with all three dispenses, well must be cleared.
Follow procedure for Clogged Probes. If inconsistent and lower vol-
ume has occurred on any of the three dispenses, perform an Empty
Head Procedure for a reverse flush of wash head.

Clogged Probes

Probes have the potential of being clogged by small particles from the wash
liquid, crystallization, or protein build-up. It is important to perform Daily and
Weekly maintenance to keep probes free of debris.
Step 1 Perform Daily Maintenance to rinse system.
Step 2 Perform Weekly Maintenance to clean probes.
Step 3 Clear particles from probes.
Identify which probe is clogged.
Use a probe wire to clear the probe.
Step 4 Replace Rinse reservoir bottle with reservoir bottle filled with dis-
tilled water.
Step 5 Verify waste tubing is connected to vacuum port and pump is on.
Step 6 Perform a forward flush to remove the particles.
Insert a clean microplate into the left magazine.
Press the
Press the
Repeat 3 times.
31
key to enter the Rinse/Prime mode.
[Rinse]
key a second time to start the prime program.
[Start]
SkanStacker 300 User Guide