Application Tips: Skanwasher 300/ Skanstacker - Molecular Devices SkanStacker 300 User Manual

Troubleshooting

Application Tips: SkanWasher 300/ SkanStacker

Instrument Functions
Air Pressure
Internal pressure pump creates air pressure within the liquid reservoir.
Air pressure increases the flow rate and has no effect on volume.
Increase the air pressure for higher pressure, decrease for lower
pressure.
Internal pressure pump adjusted through Edit Program through the Setup
Menu.
Vacuum pressure
Vacuum pressure is regulated from pump gauge.
Vacuum pressure determines the rate of aspiration.
Increasing the vacuum pressure value decreases the vacuum.
To convert mm Hg to mbar pressure, consult conversion table at web site:
http://www.info@speckdesign.com.
Suggested setting
These values are suggested as a starting point for your assay. Test between
adjustments to optimize air and vacuum pressure for each specific assay.
Applications
ELISA and
adherent cells
Cells
45
External
Vacuum Air Pressure
pressure Range 0.0-0.6
setting at bar
pump (Edit Program)
250 mbar
350 mbar
Software Setting
Set Aspirate
Position
Range 0-6 mm
(Edit Program)
0.25 bar
(0=bottom of
0.15-0.20 bar
SkanStacker 300 User Guide
0-2 mm
plate)
2-4 mm