Chapter 4: Software Configuration - Molecular Devices SkanStacker 300 User Manual

Chapter 4: Software Configuration

The SkanStacker allows up to eight different user-defined and programmed
wash sequences to be stored in memory with a battery backup. Each wash
program has the following options:
Air pressure from the internal pressure pump can be varied to control
inlet liquid flow.
Volume adjustment conversion factor, σV, can be used to fine-tune
the dispense volume to adjust for the different viscosities of various
liquids.
The position (height) of the aspirate probe above the bottom of the
well during aspiration can be varied. The amount of fluid kept in the
well can be adjusted.
Dispense probe height position in the well during washing can be set.
Up to 16 wash sequence steps can be selected. Each step can be one
of the following:
Aspirate, time in seconds [2–10 sec.]
Wash, time in seconds [0.1–10 sec.]
Wash, volume in µl [100–900 µl]
Dispense, volume in µl [100–350 µl]
Wash from inlet Rinse, time in seconds [0.1–10 sec.]
Soak/pause, time in minutes and seconds [1 sec.–2 min 50 sec.]
The washer is pre-programmed with four typical wash sequences. These may
be removed or changed as necessary.
At the end of this Guide, a blank program guide sheet is included to facilitate
programming. Duplicate this sheet for your own use to save a hard copy of
each program for reference purposes.
SkanStacker 300 User Guide
Software Configuration
14