Chapter 1: Introduction; Overview - Molecular Devices SkanStacker 300 User Manual

Overview

The need to thoroughly wash unbound antigens, antibodies, binding proteins,
enzymes, or receptors from a solid-phase binding support is one of the most
important steps in the enzyme-linked immunosorbent assay (ELISA). The
SkanStacker 300 meets this demand and those of other numerous laboratory
assays now being performed in 96-well microplates where thorough washing
is an essential component of the assay.
The SkanStacker is designed to wash 96-well microplates. The process of set-
ting up wash parameters is flexible and allows you to modify or choose the
following settings:
Aspirate probe height and position in the well for low residual
volume.
Dispense probe height and position in the well for washing the active
part of well.
Inlet wash buffer flow rate can be adjusted by changing air pressure.
Range 0.00 - 0.60 bar in selected programs.
"Overflow" washing with meniscus.
Microprocessor-controlled timing.
Aspiration from the top of the well with adjustable lift speed
controlled by adjusting aspirate time in seconds.
Wash all 96 wells simultaneously.
Dispense-only function from 100 -350 µl.
Dispense volume adjustment.
Adjustable vacuum pump with manometer.
Electronic air pressure regulation.
Automatic handling of up to 25 microplates.
SkanStacker 300 User Guide

Chapter 1: Introduction

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