CellScale BioTester User Manual page 82

To transversely adjust the goosenecks, loosen the fasteners that attach the goosenecks to the
actuators, slightly rotate the gooseneck, and retighten the fasteners.
should be helpful as a guide. The goal is to make the rake tines of opposing BioRakes collinear.
To axially adjust the goosenecks, use the actuator jog icons in the lower left of the screen. Make
sure the "independent" radio button is selected and not the "mirror matching" button. The jog
speed should be about 7. The goal is to make a square as shown in the figure above.
Adjust all 4 goosenecks until you are satisfied with their transverse and axial alignment and the
rake tips form a square as shown above. It may also be necessary to make small adjustments to
the camera position. The camera alignment should be parallel to the rake alignment and the
BioRake tips should be in the middle of the image.
It is now necessary to perform a center position calibration.
temporarily define the current position as the center position. This calibration can be performed
in the following steps:
1. Lower the fluid chamber and remove the BioRakes.
2. From the Tools menu select Advanced and then select Centre Position Calibration. This
should bring up this dialogue box:
3. Press Yes.
4. Select the Reset Actuators button
5. From the Tools menu select Advanced then select Move to Centre. This should bring up
this dialogue box:
6. Press Yes. Once the actuators have stopped moving, put 2 opposing BioRakes on the X
axis.
7. Using the actuator jog control arrows at the lower left of the screen, move the actuators
until all 10 BioRake tips are collinear.
selected for this step and not the "independent" button! Note that it is the tips of the
BioRakes that should be collinear and not the outside edge. Because the tips are
underneath the BioRakes and cannot be seen on screen, it is necessary to estimate their
position. Please use the image below as a reference:
First, it will be necessary to
(or use Tools>Reset Actuators).
Make sure the "mirror matching" button is
BioTester User Manual
The on-screen image
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