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CellScale BioTester 5000 User Manual

Biaxial test system
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BioTester
5000
Biaxial Test System
User Manual
version 8.6

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Summary of Contents for CellScale BioTester 5000

  • Page 1 BioTester 5000 Biaxial Test System User Manual version 8.6...
  • Page 2 © 2024 CellScale. All rights reserved. This material may not be reproduced, displayed, modified or distributed without the express prior written permission of the copyright holder. For permission, contact CellScale Biomaterials Testing at info@cellscale.com.

  • Page 3: Table Of Contents

    Table of Contents General Information ....................... 1 Environmental and Electrical Specifications ................1 System Assembly ........................1 Power Connections ........................1 Safety Warnings ........................1 Approvals and Certification ....................... 1 Manual Operating Controls ....................... 2 Software ........................... 2 General Maintenance........................ 2 Testing Terminology ......................
  • Page 4 11. Appendix A: Initial System Setup ..................46 Tools ............................46 Fasteners & Parts ........................46 Unpack System ........................47 Remove the BioTester 5000 from the shipping container .............47 Attach Camera Mast ........................49 Place Fluid Chamber on Riser Stage ..................51 Install Camera Lens .........................52 12.
  • Page 5 Stow Cables and Plug in the Load Cells ..................67 Attach the Temperature Probe ....................69 Attach the Knob to the Pull Rod ....................69 Turn on the BioTester 5000 .....................69 Launch the LabJoy Software ....................70 Perform Camera Alignment......................73 Perform Vertical Gooseneck Alignment..................76 Perform Transverse and Axial Gooseneck Alignment ..............78...

  • Page 6: General Information

    BioTester 5000 User Manual 1. General Information The BioTester 5000 is a precision test instrument specifically designed for the measurement and analysis of small biological specimens. The system includes a compact biaxial test station and an integrated software interface to run and analyze test results. The system is intended for indoor use only.

  • Page 7: Manual Operating Controls

    Software LabJoy software is included and is required to run the BioTester 5000. 2 x USB ports are required for data connections, with at least one of those ports being USB 3.0. Windows 10 or higher is required to run LabJoy.

  • Page 8: Testing Terminology

    BioTester 5000 User Manual 2. Testing Terminology The BioTester 5000 is designed to apply biaxial forces to soft tissue specimens (deforms by more than 1% in vivo) with an in-plane dimension of 3 - 15 mm. This includes biological material such as eye tissue, heart valves, pericardium, joint capsules, large blood vessels, scaffolds, and soft polymers.

  • Page 9: Phases, Cycles, And Test Sequences

    BioTester 5000 User Manual Phases, Cycles, and Test Sequences As the following diagram demonstrates, each application and release of load on the specimen is called a test cycle. The same test cycle can be repeated multiple times to achieve a certain goal (preconditioning, physiological conditioning, or testing);...

  • Page 10: Test Phases: The Smallest Unit Of Testing

    Force control can be applied as a ramp or sinusoid function. In addition, strain control from image feedback is available for the BioTester 5000. This control mode utilizes displacements based on a region within a specimen rather than global actuator displacements.

  • Page 11: Control Functions

    BioTester 5000 User Manual Control Functions The BioTester 5000 makes it possible to test specimens under several control functions: Under Displacement or Force control: The ramp function applies the displacement or force at a constant nominal rate. This is equivalent to a constant engineering strain or constant velocity.

  • Page 12: System Overview

    BioTester 5000 User Manual 3. System Overview System Components The BioTester 5000 brings together several high-precision components, making it the state-of- the-art instrument for testing biomaterials. High-resolution CCD camera provides synchronized video tracking and analysis. Illumination and lens provided to capture excellent...

  • Page 13: Software Overview

    BioTester 5000 User Manual Software Overview The software included with the BioTester 5000 is called LabJoy. It is divided into two modules, a data collection module and a review and analysis module. The data collection module is used to set test parameters, enable specimen loading and testing, and monitor test progress. The screen...

  • Page 14: Output Files And Data Structures

    BioTester 5000 User Manual Output Files and Data Structures For each test, the BioTester 5000 creates and saves three file types. The following table describes the three file types for a project named “Sample1”. Output from this test would be found in a “Sample1”...

  • Page 15: Setting Up & Starting A Test

    BioTester 5000 User Manual 4. Setting Up & Starting a Test Overview Setting up and running a new test is a simple process made up of both mandatory and optional steps. The following list presents all the steps, while the rest of this chapter describes each of the steps in detail.
  • Page 16 The system uses the last location selected as the default. BioTester 5000 Tip: Selecting and Using Templates How to select a template: Designing an appropriate test sequence is an art that depends on both the type of material being tested and the specific material properties you are interested in measuring.

  • Page 17: Step 2: Reset The Actuators

    By resetting the actuators, you are moving the actuators to the precise home position ensuring that the displacement measurements taken by the BioTester 5000 are accurate. To reset the actuators, select Reset Actuator from the tools menu, or press on the toolbar.

  • Page 18: Step 4: Zero The Load Cells (Occasionally)

    BioTester 5000 User Manual Step 4: Zero the Load Cells (occasionally) While it is not necessary to zero the load cells with every test sequence, we suggest zeroing the load cells at the start of a new test session. With repeated use, the zero point of the load cells can drift.
  • Page 19 BioTester 5000 User Manual Test Parameter Description Control Mode Biaxial tests are typically performed under displacement control; however, you can select the following control modes to achieve specific testing objectives: • To obtain uniaxial properties for a biaxially mounted specimen, specify displacement control in one axis and Force control with zero Load Magnitude in the other axis.

  • Page 20: Step 6: Mount The Specimen

    BioTester 5000 User Manual Step 6: Mount the Specimen Proper specimen mounting is critical to ensuring accurate and repeatable results. It is best to have the tines spaced evenly across the edge of the specimen and as closely to the edges as possible.
  • Page 21 BioTester 5000 User Manual...

  • Page 22: Step 7: Execute The Test

    BioTester 5000 User Manual BioTester 5000 Tip: Ideal Specimen Size The BioTester 5000 is designed for small specimen sizes. For Standard BioRakes (5 tines spaced at 1 mm) the ideal specimen size is square with side lengths of 5.5 - 6 mm.

  • Page 23: Step 9: End Of Test Controls (Optional)

    BioTester 5000 User Manual Step 9: End of Test Controls (optional) Once the test has been completed, you can choose to start a new test or use the system controls by selecting the “OK” button. System controls include the top ribbon as well as Actuator Control. These controls can be useful...

  • Page 24: Strain Control From Image Tracking

    BioTester 5000 User Manual 5. Strain Control from Image Tracking Strain control from image tracking is an alternate test control mode that can specify and control the test displacements based on a region within a specimen rather than the global actuator displacements.

  • Page 25: Specify Testing Parameters

    BioTester 5000 User Manual Specify Testing Parameters To use strain control: 1. Double click or select Edit on the desired protocol line to open Set Parameter Editor 2. Select Strain From Image as the Control Mode. This mode will only be available if a scientific camera set up is connected.

  • Page 26: Selecting The Tracking Point Locations

    BioTester 5000 User Manual Selecting the Tracking Point Locations A test protocol that is using strain control with image tracking will display a rectangle in the live video feed as follows: The small squares are the points that will be tracked. The location of the tracking points can be adjusted by clicking and dragging the corners of the box or the center tracking points on each side.

  • Page 27: Configuring Output Data Files

    BioTester 5000 User Manual Configuring Output Data Files Measured and Nominal strains are available as test output columns for the csv data file. From the Settings menu, select Data Output. Then click the Configure button to select the desired output columns.

  • Page 28: Executing The Test

    BioTester 5000 User Manual Executing the Test Upon executing a test, the dialog box shown below will appear. It gives options to automatically adjust camera settings and to automatically adjust tracking point locations. Optimized lighting and camera settings and adjusted tracking points locations may improve the success of tracking.

  • Page 29: Advanced Controls

    BioTester 5000 User Manual Advanced Controls Strain control from image tracking is controlled with a PID control algorithm. The PID control parameters can be adjusted by selecting Motion Control from the Settings menu in the top ribbon. Check the Edit Advanced Settings...

  • Page 30: Additional Settings

    BioTester 5000 User Manual 6. Additional Settings Configuring Output Data Files From the Settings menu, select Data Output to display the dialogue shown on the left. Click the Configure button to select which of the following columns to output. →...

  • Page 31: Advanced Settings

    BioTester 5000 User Manual Advanced Settings Advanced settings are available by selecting Advanced from the Settings menu. All of these settings are template specific. Show Warnings By selecting the check boxes, a warning dialogue will appear before either action button (i.e.

  • Page 32: Motion Control Settings

    BioTester 5000 User Manual Motion Control Settings Motion Control Settings affect the rate and stability at which forces are achieved in force- controlled procedures. These settings apply to preloads as well as Force Control Modes. Changes to these settings are saved to the test template.

  • Page 33: Acceleration

    BioTester 5000 User Manual Acceleration Acceleration setting can be set as an integer value between 1 and 10 (see chart below for corresponding acceleration values). Changes made to the acceleration will affect tests under both force and displacement control. Reducing this value may help smooth force-controlled tests under low loads or displacement-controlled tests at high speeds.

  • Page 34: Control Algorithm Settings Editing (Advanced)

    We recommend using the adaptive algorithm that has already been optimized for the device, as improperly tuned PID settings may result in unstable behavior. We suggest only advanced users with specialized tests modify these PID settings. Contact CellScale for more information regarding these settings.

  • Page 35: Range Limits

    BioTester 5000 User Manual Range Limits Range limits can be accessed by selecting Range Limits from the Settings menu. Soft Limits To avoid rake collisions or sample destruction, it is useful to assign reasonable travel limits to the actuators. This is especially important during force...
  • Page 36 BioTester 5000 User Manual Graph Data Force versus Time This graph shows how the measured X and Y forces are changing with time. Peak loads per cycle, differences in load between the X and Y axes, and force relaxation are all easily seen in this type of graph.

  • Page 37: Reviewing Test Results

    BioTester 5000 User Manual 7. Reviewing Test Results The BioTester 5000 includes an additional software, Data Analysis, to review and analyze data collected from the device. This includes graphing, curve fitting, material modeling and image processing to determine detailed strain maps.

  • Page 38: System Hardware Settings

    BioTester 5000 User Manual 8. System Hardware Settings Controller The BioTester 5000 uses an integrated control board rather than a group of PC controlled device drivers. This minimizes communication lag and allows the device to operate asynchronously with the PC. The...
  • Page 39 BioTester 5000 User Manual Changing Camera Image Resolution The default resolution of the camera is 2048x2048, the current image resolution is written at the bottom of the screen. If you would like to capture images at a higher frequency (25Hz or 50Hz) you can reduce this resolution to 1024x1024.

  • Page 40: Load Cells

    BioTester 5000 User Manual Load Cells The load cells are semiconductor strain gage-based with mechanical overload protection (for the lower capacity load cells). They have an accuracy equal to 0.2% of the rated full scale load. The load cells are available in 0.5N, 1.5N, 2.5N, 5N, 10N and 23N sizes.

  • Page 41: Heater

    Heater To activate the fluid chamber heater, both power switches on the front of the BioTester 5000 need to be in the On position (the computer and software do not need to be on yet). At this point, the heater will warm the fluid to the last used temperature set point.

  • Page 42: External Sync Pulse

    BioTester 5000 User Manual External Sync Pulse A 5-volt TTL external sync pulse signal can be generated on BioTester 5000 models containing a BNC connector jack on the back panel. The sync pulse will be pulsed on the very first sample pulse, which occurs when the load cells are measured and the motor positions of all 4 axes are read.

  • Page 43: System Calibration And Advanced Tools

    BioTester 5000 User Manual 9. System Calibration and Advanced Tools Load Cell Calibration Check If the load cells are changed, moved, or impacted, it is good practice to perform a load cell calibration check. The calibration check procedure is as follows: 1.
  • Page 44 BioTester 5000 User Manual 4. Lower the fluid chamber and install the calibration spring brackets on the goosenecks. 5. Zero the load cell using the Zero Load Cells button. 6. Using the Jog+ and Jog– buttons, drive the actuators until the posts are correctly spaced so that the calibration spring can be installed without applying any significant load.
  • Page 45 BioTester 5000 User Manual 9. Click the Run button, wait for calibration to complete. 10. Once the calibration procedure is complete, a dialog window will appear displaying the relative % difference. This value reflected the difference between the factory calibrated factor and the calculated factor from the calibration procedure.

  • Page 46: Legacy Load Cell Calibration (Type P)

    BioTester 5000 User Manual Legacy Load Cell Calibration (Type P) Load cells marked as Type P were factory calibrated with a different procedure. These types of load cells require calibration if they are changed, moved, or impacted. The load cell information and type is displayed in the log window in the bottom left corner of the Labjoy software.

  • Page 47: Snap Image Feature

    The BioTester 5000 comes equipped with a 10X zoom lens as shown below. Other versions of the BioTester 5000 may be equipped with a fixed magnification 75mm focal length lens (see Appendix E: Fixed Focal Length Lens).
  • Page 48 BioTester 5000 User Manual Using this lens, it is possible to achieve a wide ranging field of view as shown in the graph below. Possible values for field of view are shown by the shaded gray region of this graph and can range from 5.5mm to 24mm.

  • Page 49: Update Firmware

    BioTester 5000 User Manual Update Firmware Periodic firmware updates may be issued by CellScale for the BioTester. Updates are available at https://www.cellscale.com/downloads/. The device firmware will automatically update as part of the software installer if your device is connected via USB and powered on.

  • Page 50: Troubleshooting

    When a communication error occurs, close LabJoy, check connections, power cycle the BioTester 5000, and restart LabJoy. BioTester 5000 Tip: Preventing Power Disruptions We recommend plugging the BioTester 5000 (and host computer) into an uninterruptable power supply (UPS) or battery back-up.

  • Page 51: Appendix A: Initial System Setup

    BioTester 5000 User Manual 11. Appendix A: Initial System Setup The BioTester 5000 has been packed in a special container to protect it during shipping. In addition, system accessories have been packed in several cartons contained in a separate box.

  • Page 52: Unpack System

    Carefully remove the protective shipping material from the container such that the BioTester 5000 can be lifted out. Remove the camera mast first then the BioTester 5000 main unit. In addition, protective foam will have to be removed from the main unit and camera mast after lifting out the...
  • Page 53 BioTester 5000 User Manual...

  • Page 54: Attach Camera Mast

    BioTester 5000 User Manual Attach Camera Mast Thread the camera post into the receiver. The knurled collar must be rotated to fasten the camera post. It will take many turns but eventually the mast will lock into place. Try to position the camera approximately as shown, although the final position can be adjusted later in the procedure.
  • Page 55 Once the camera mast is in place, the cables for the lights and camera USB will be routed out the back of the mounting pole. The camera USB can be plugged into the PC and the connector for the lights can be plugged into the back panel of the BioTester 5000.

  • Page 56: Place Fluid Chamber On Riser Stage

    BioTester 5000 User Manual Place Fluid Chamber on Riser Stage Put the fluid chamber in place on the riser stage and align the temperature probe mounting hole with the temperature probe cable.

  • Page 57: Install Camera Lens

    BioTester 5000 User Manual Install Camera Lens Remove the protective caps on the lens and camera body and then screw the lens onto the camera. The correct camera setup can be fine-tuned at a later step. If using a fixed focal length lens (typically 75mm), see Appendix E for installation.

  • Page 58: Appendix B: Software Installation

    A Windows based PC (Windows 10 or higher) with at least 2 USB ports (1x USB 3.0 minimum) is required to operate the BioTester 5000. A label on the cover of the manual contains a web address to download the LabJoy installation package. These files contain the software installer for LabJoy, which is the user interface and control software for the BioTester 5000.
  • Page 59 BioTester 5000 User Manual If your BioTester is detected this screen will popup: If BioTester is not detected, then you will be taken to a model selection screen where you can select your BioTester model. To install the correct user manual and templates please select the...
  • Page 60 BioTester 5000 User Manual...
  • Page 61 BioTester 5000 User Manual...
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  • Page 63: Appendix C: Install Load Cells / Perform Camera And Gooseneck Alignment

    Connect the camera USB cable to a PC USB port. Connect the included USB cable to the main unit and a PC USB port. Plug in the power supply to the BioTester 5000 and then proceed with the following steps. Do not turn the BioTester 5000 on yet.
  • Page 64 BioTester 5000 User Manual Attach the load cell stud to the load cell using your fingers. The load cell stud has one side with a shoulder below the threads which should be attached to the load cell. The other end is attached to the gooseneck.
  • Page 65 BioTester 5000 User Manual Attach the other end of the load cell stud to the gooseneck. Finger tighten a 6-32 nut onto the threaded stud. The load cell cable should be at about a 45° angle to the gooseneck as shown...
  • Page 66 BioTester 5000 User Manual The load cell and gooseneck assembly should look like this when assembled. There are two styles of load cells available (both are shown below). For 10N and 23N load cells, ensure the included spacer is attached before mounting it to the actuator bracket. This is important for proper spacing between the goosenecks.
  • Page 67 Check that all components are snugly in place by gently turning the base of the load cell against the gooseneck. All components should only be finger tight while being snug enough so that the components don’t freely rotate while using the BioTester 5000.

  • Page 68: Attach The Load Cell Brackets To The Actuators

    BioTester 5000 User Manual Attach the Load Cell Brackets to the Actuators Before attaching the load cell/gooseneck assembly, ensure the load cell brackets are installed on the appropriate actuators. Use M4 x 10mm (x4) fasteners provided for each bracket as shown below.

  • Page 69: Attach The Goosenecks To The Actuators

    BioTester 5000 User Manual Attach the Goosenecks to the Actuators Attach each load cell/gooseneck assembly to the load cell bracket on the appropriate linear actuator using a 6-32 nut. The load cells are attached to the actuators on either side of the camera post.
  • Page 70 BioTester 5000 User Manual Attach the 2 remaining goosenecks to the appropriate linear actuators using 4-40 fasteners (2 each).
  • Page 71 BioTester 5000 User Manual...

  • Page 72: Stow Cables And Plug In The Load Cells

    The initial length of cable should bend towards the outside edge of the BioTester 5000 as shown. Position the cables inside the cover so that they do not interfere with the actuator carriage travel, particularly during actuator reset where the carriages are fully retracted.
  • Page 73 BioTester 5000 User Manual...

  • Page 74: Attach The Temperature Probe

    Attach the temperature probe and place it in the sensor pocket in the fluid chamber. Attach the Knob to the Pull Rod A small knob screws into the pull rod at the front of the BioTester 5000. This pull rod is used to raise and lower the stage.

  • Page 75: Launch The Labjoy Software

    BioTester 5000 User Manual Launch the LabJoy Software Launch the LabJoy software. The default screen should appear as: Selecting File>Collect New should bring up this window:...
  • Page 76 Select OK. If prompted to Reset Actuators, select “Yes”. If prompted to Calibrate Load Cells, select “No”. Next place a standard size BioRake (1.0mm spacing, blue caps) in each of the 4 goosenecks so that the BioTester 5000 looks like this:...
  • Page 77 BioTester 5000 User Manual On the LabJoy control screen, ensure that the specimen size is set to 5000µm in X and Y and then click the Move to Size button...

  • Page 78: Perform Camera Alignment

    BioTester 5000 User Manual Perform Camera Alignment The camera position has both coarse and fine position adjustments and coarse and fine focus adjustments. To start, loosen the fasteners which tighten to camera cover to the mast. Position the camera cover vertically such that the working distance from the lens surface to the BioRakes is 15cm.
  • Page 79 BioTester 5000 User Manual Attempt to bring all of the BioRakes into the image, as shown below: Next, fine tune the camera position using the knobs at the back and side of the camera housing and fine tune the image focus using the lowest ring on the lens.
  • Page 80 BioTester 5000 User Manual Iris (fully open) Zoom Focus Adjustments to the zoom can be made by adjusting the middle ring on the lens. See the section on “Adjusting the Camera Position and Image Magnification” in section 8 of this manual for more...

  • Page 81: Perform Vertical Gooseneck Alignment

    BioTester 5000 User Manual Perform Vertical Gooseneck Alignment Because the goosenecks attached to the load cells are connected by only 1 threaded stud, the vertical and transverse alignment of the end of the gooseneck can be somewhat different each time the goosenecks are removed and reattached. To accommodate the vertical variation, shims between the gooseneck brackets and the actuators may need to be added or removed.
  • Page 82 BioTester 5000 User Manual By looking at the BioRakes from the side, it is possible to observe their vertical alignment. Please add or remove shims to vertically align the BioRakes. Here are photos of poor vertical alignment and good vertical alignment:...

  • Page 83: Perform Transverse And Axial Gooseneck Alignment

    BioTester 5000 User Manual Perform Transverse and Axial Gooseneck Alignment This step requires 2 different adjustment mechanisms. The goal is to make the BioRakes look like this: First, make sure the BioRakes are positioned in the transverse center of the gooseneck. The magnetic mounts that hold the BioRakes to the gooseneck fix them in the axial direction, but allow a small amount of adjustment in the transverse direction.
  • Page 84 BioTester 5000 User Manual To axially adjust the goosenecks, use the actuator jog icons in the lower left of the screen. Make sure the “independent” radio button is selected and not the “mirror matching” button. The jog speed should be about 7. The goal is to make a square as shown in the figure above.
  • Page 85 BioTester 5000 User Manual Example 1: BioRakes have too much overlap. Example 2: BioRakes are correctly positioned.
  • Page 86 BioTester 5000 User Manual Example 3: BioRakes do not have any overlap so tips are not collinear. 8. Remove the BioRakes and place them on the Y axis. 9. Using the actuator control arrows, drive the Y actuators until all 10 BioRake tips are collinear as in step 4 (IN MIRROR MATCHING MODE ONLY!).

  • Page 87: Install Actuator Covers

    BioTester 5000 User Manual Install Actuator Covers Each actuator cover has a number written on the inside. These numbers match up with numbers written on the inside of the main cover to ensure the best possible fit between the actuator covers and the main cover.

  • Page 88: Appendix D: Mechanical Grip Usage

    BioTester 5000 User Manual 14. Appendix D: Mechanical Grip Usage In addition to BioRakes, the BioTester 5000 can optionally be equipped with mechanical grips for sample attachment. Two types of grips are available: Screw Close (Stainless Steel) and Spring Close (Plastic).
  • Page 89 BioTester 5000 User Manual 3. Jog the actuators to fit the size of the specimen. 4. Place the specimen on the grip face and tighten the fastener. Be careful to not apply excessive downward force on the screw!

  • Page 90: Spring Close Grips (Plastic)

    BioTester 5000 User Manual Spring Close Grips (Plastic) The spring close grips can be equipped with three different springs of varying stiffness to exert different gripping forces. These springs are provided with the grips and may be switched at any time using the provided hook tool.

  • Page 91: Sample Mounting

    BioTester 5000 User Manual Sample Mounting Squeeze the grip levers to open the jaws of the grip. Slide the end of a test specimen between the jaws and gently release the levers. Specimens may be mounted and tested using 2 grips (uniaxial) or 4 grips (biaxial).
  • Page 92 BioTester 5000 User Manual Figure 1 - Uniaxial Test Setup Figure 2 - Biaxial Test Setup...

  • Page 93: Changing Grip Springs

    BioTester 5000 User Manual Changing Grip Springs 1. Using the hook tool provided, pull on the spring and remove the metal dowel holding the grip spring in place. After this step, the spring may be slowly released. System Alert A fully installed spring experiences a high amount of tension. When the springs are disengaged, be sure not to let the hook tool slip or the spring may be released at high velocity.
  • Page 94 BioTester 5000 User Manual 2. Replace the old spring with a new spring inside the mounting hole. 3. Secure one side of the spring onto the grip with the metal dowel 4. Use the hook tool to pull the other side of the spring into place and secure it with another...

  • Page 95: Appendix E: Fixed Focal Length Lens

    15. Appendix E: Fixed Focal Length Lens In addition to the 10X zoom lens described in Section 8, the BioTester 5000 system can also use fixed focal length lenses. Other BioTester 5000 models may have a 75mm focal length lens and a set of lens spacers.

  • Page 96: Adjusting Camera Position And Image Magnification

    BioTester 5000 User Manual Adjusting Camera Position and Image Magnification The camera position can be adjusted using the knobs at the back and side of the camera housing. If larger position adjustments are needed, the bolts that attach the camera to the mast can be loosened to allow rotation of the camera housing.
  • Page 97 BioTester 5000 User Manual 25mm (NT56-529) 35mm (NT56-530) 50mm (NT56-531) 75mm (NT56-532) 100mm (NT56-675) Stand Stand Stand Stand Stand Spacer pix/c pix/c pix/c pix/c pix/c (mm) (mm) (mm) (mm) (mm) (mm) (mm) (mm) (mm) (mm) (mm) 685 78.6 162.8 1070 71 179.3...

  • Page 98: Appendix F: Protocol For Tying And Cutting Sutures For Biaxial Testing

    16. Appendix F: Protocol for Tying and Cutting Sutures for Biaxial Testing In addition to BioRakes and grips, the BioTester 5000 can optionally be equipped with bracket and pulley system to attach samples using sutures. There are many ways to attach specimens to the suture brackets using a variety of suture designs.
  • Page 99 BioTester 5000 User Manual 2. Bend suture hooks...
  • Page 100 BioTester 5000 User Manual 3. Tie off sutures. First hook each bent hook around a secured post held in the vice. Then tie a standard knot in the suture and use forceps to guide the location of the knot so that...
  • Page 101 BioTester 5000 User Manual...
  • Page 102 BioTester 5000 User Manual 4. Use pliers to tighten knot...
  • Page 103 BioTester 5000 User Manual 5. Cut off excess.
  • Page 104 BioTester 5000 User Manual The resulting mounted specimen should ideally look something like this: © Copyright 2024 CellScale Biomaterials Testing.

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