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CellScale MechanoCulture TM User Manual

Mechanical stimulation system
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MechanoCulture TM
Mechanical Stimulation System
User Manual
version 1.1

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Summary of Contents for CellScale MechanoCulture TM

  • Page 1 MechanoCulture TM Mechanical Stimulation System User Manual version 1.1...
  • Page 2 CellScale has systems optimized for high-throughput, real-time imaging, and 3D scaffold use. In addition to our standard products, CellScale can also develop a custom solution for your application. Our products are used by researchers around the globe to study everything from tissue strength and stiffness to cellular response.

  • Page 3: Table Of Contents

    MechanoCulture TM User Manual Table of Contents General Information ......................... 1 Environmental and Electrical Specifications ................1 System Assembly......................... 1 Connections to Supply ......................... 1 Safety Warnings ........................... 1 Manual Operating Controls ......................1 General Maintenance ........................1 Approvals and Certification ......................1 Components Overview......................
  • Page 4 MechanoCulture TM User Manual 10. Appendix C: System Assembly ....................22...

  • Page 5: General Information

    MechanoCulture TM User Manual 1. General Information The MechanoCulture TM is a precision instrument specifically designed for deforming flexible membranes in a sterile fluid environment. The device has an onboard actuator and a detached control board to enable it to run independently of a computer in an environment-controlled incubator.

  • Page 6: Components Overview

    MechanoCulture TM User Manual 2. Components Overview Mini USB Port Control Box Power Input Port 3.5mm TRRS Cable Jack Start/Stop/Reset Button...

  • Page 7: Testing Terminology

    MechanoCulture TM User Manual 3. Testing Terminology Multiphase Test Cycles In order to properly mechanically stimulate/signal cells, it is often necessary to apply more than one load magnitude or frequency during the course of the test. Phases, Cycles, and Test Sequences As the following diagram demonstrates, each application and release of the well plate is called a test cycle.

  • Page 8: Test Phases: The Smallest Unit Of Testing

    MechanoCulture TM User Manual Test Phases: The Smallest Unit of Testing The test phase is the smallest unit of the test specification. There are four phases within a cycle. Each phase serves a specific purpose: Stretching - During the stretch phase, a deformation is applied to the specimen.

  • Page 9: Software Overview

    MechanoCulture TM User Manual 4. Software Overview The MechanoCulture software is used to program the device with the phases, cycles, and sets that comprise the test sequence. The screen layout is shown below: Test Parameters Message Window Step 1: Initiate a Test Sequence Test sequences can be initiated in 3 different ways: 1.

  • Page 10: Step 2: Modify Testing Parameters (Optional)

    MechanoCulture TM User Manual Step 2: Modify Testing Parameters (optional) You can select and modify parameter sets by clicking on their row in the Test Parameter Specification table and then pressing the Edit Set button (or by double clicking on their row). When you do so, the Set Parameter Editor Dialog will appear.

  • Page 11: Well Plate Pre-Stretch, Device Reset Movement, And Offset Distance

    MechanoCulture TM User Manual Well Plate Pre-Stretch, Device Reset Movement, and Offset Distance On power-up, the system’s actuator will go through a series of moves in order to achieve the correct starting position. These moves involve fully extending the actuator and then retracting to the correct load position.

  • Page 12: Offset And Jog Functions

    MechanoCulture TM User Manual Offset and Jog Functions The offset function for the MechanoCulture TM is programmable by the user and how to do this is shown later in the manual. The jog buttons let you extend and retract the actuator outside of executing a test. When the actuator is jogged, the offset value will automatically update and display the current position of the moving pins.

  • Page 13: Designing Test Sequences

    MechanoCulture TM User Manual 5. Designing Test Sequences The MechanoCulture software allows for complex test sequences to be generated. examples in this section illustrate how to generate different types of test protocols. Example 1: Continuous Cycling for Fixed Duration and then Stop Objective: Create a test sequence that cycles at 0.5 Hz with 2.6mm of stretch for 24 hours and...
  • Page 14 MechanoCulture TM User Manual The resulting actuator motion versus time graph for the first 30 seconds: Time (S)

  • Page 15: Example 2: Pre-Stretch A Single Time, Then Cycle For A Fixed Duration, And Then Stop

    MechanoCulture TM User Manual Example 2: Pre-stretch a Single Time, Then Cycle for a Fixed Duration, and Then Stop Objective: Create a test sequence that starts with a 0.5 mm pre-stretch in 1 minute followed by 100 cycles of an additional 2 mm stretch (5-minute stretch, 1-minute hold and 4-minute recover).
  • Page 16 MechanoCulture TM User Manual The resulting actuator motion versus time graph for the first 30 minutes: Time (S)

  • Page 17: Example 3: Intermittent Stretching

    MechanoCulture TM User Manual Example 3: Intermittent Stretching Objective: Create a test sequence that cycles at 1 Hz with 2mm of stretch for 10minutes followed by 50minutes of rest. Repeat this pattern indefinitely. 1. Press Insert Above to create a Set.

  • Page 18: Setting Up & Running A Test

    MechanoCulture TM User Manual 6. Setting Up & Running a Test Overview As a user of the MechanoCulture, you are likely to develop your own test preparation and execution protocol, but there are several elements that are common to most users. This manual will not cover cleaning, disinfecting, sterilization, cell seeding, reagents, and a number of other topics.

  • Page 19: Advanced Parameters

    MechanoCulture TM User Manual 7. Advanced Parameters Under the Hardware menu is an Advanced Settings menu containing several different values which should be understood. The menu will appear as below: Offset: Offset is a distance that controls the starting stretch magnitude of each test.

  • Page 20: Appendix A: Updating The Firmware

    MechanoCulture TM User Manual 8. Appendix A: Updating the Firmware 1. With the MechanoCulture connected to the PC and turned on, launch the firmware update software located in the Windows start menu under MechanoCulture>Utilities. 2. Load the firmware file using the “Load Update File” button.

  • Page 21: Appendix B: Software Installation

    MechanoCulture TM User Manual 9. Appendix B: Software Installation Included in your shipment is a link to install the MechanoCulture software. This installation package contains the installer for the software to program your MechanoCulture devices. To install the software, go to the provided web address and download the compressed software package.
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  • Page 26 MechanoCulture TM User Manual 10. Appendix C: System Assembly Follow these steps to assemble your MechanoCulture TM system for use: 1. Cut the membrane material to 2.5” wide to the desired length (100mm for the default specimen) and gather the following tools: a.
  • Page 27 5. Plug in the controller and main unit and reset the actuator. 6. Mount the specimen and loading rings assembly to the corresponding locations on the main unit. Press the start button on the controller to initiate the test. © Copyright 2022 CellScale Biomaterials Testing.

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