Sutter Instrument XenoWorks BRM Operation Manual page 35

Problem
The micropipette has been
lost from the field of view.
XENOWORKS MICROMANIPULATOR SYSTEM (TOUCHDECLUTCH) OPERATION MANUAL – REV.
Cause
4. The swing gate
thumbscrew is loose.
5. The mechanical is not
screwed securely to the
mechanical base plate.
6. The mechanical base
plate is not locked to the
microscope adapter.
7. The micromanipulator is
at the extreme limit of
one or more of its axes.
8. The cable attached to
the mechanical is under
tension.
9. The center fitting of the
micropipette holder is
loose.
A too-coarse range of
movement setting has been
selected, or the joystick
resistance is too loose.
Solution
Tighten the swing gate
thumbscrew.
Ensure all four screws fixing
the mechanical to its base
plate are tight.
The mechanical base plate is
not locked to the microscope
adapter.
Remove the micropipette
holder from the
micromanipulator. Activate
the "Setup" function, which
centers the motors. If
necessary, adjust the
mechanical base plate with
respect to the microscope
adapter so that the
microscope objective
intersects the center of the
micromanipulator's Y-axis.
Realign the micropipette
holder clamp arrangement (X-
and Z-axes), so that when the
micropipette holder is
replaced in the clamp, the
micropipette tip will come as
close as possible to the
microscope's optical axis.
Only now, should the joystick
be used to fine-position the
tip of the micropipette in the
microscope field of view.
Release any tension by coiling
the cable once on the bench
behind, or to the side of the
microscope.
Tighten the fitting.
Select a finer range setting
(Speed) or tighten the joystick
friction dial.
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