Sutter Instrument P-97 Manuals

Manuals and User Guides for Sutter Instrument P-97. We have 2 Sutter Instrument P-97 manuals available for free PDF download: Operation Manuals, Operation Manual

Sutter Instrument P-97 Operation Manuals (66 pages)

Flaming/Brown Micropipette Puller

Brand: Sutter Instrument | Category: Laboratory Equipment | Size: 1 MB

Disclaimer
5
Safety Warnings and Precautions
5
1 General Information

11
- What's New on the P-97
  11
- Glass Capillary & Heating Filament Specifications
  12
  - Glass Capillary
    12
  - Heating Filament
    12
- Technical Support
  12
- System Description - Front Panel
  12
  - Figure 1-1. P-97 Front Panel
    12
  - Controls
    13
  - Display
    13
  - Figure 1-2. Program Display
    13
- System Description - Mechanical (Puller Anatomy)
  15
- Cabinet
  18
  - Electronics
    18
2 Installation

19
- Unpacking
  19
- Setting up
  19
3 Operating Instructions

21
- First Time Use
  21
- Figure 3-1. Loading a Piece of Glass
  21
- Programs
  22
- Software Control Functions
  26
- Pulling Pipettes
  30
- Parameter Adjustment
  31
  - General Information
    31
  - Figure 3-8. Sample Program
    31
  - Figure 3-9. Pull Cycle Report (Multiple-Loops)
    31
  - Micropipette/Microinjection Needle Fabrication
    32
    
    Heat
    32
    
    PULL Strength
    32
    
    VELOCITY (Trip Point)
    32
    
    Delay Mode (Cooling)
    33
    
    TIME Mode (Cooling)
    33
    
    Filament Width
    34
    
    PRESSURE Adjustment
    34
  - Patch Pipette Fabrication
    34
    
    Heat
    34
    
    Cooling
    35
    
    Pressure
    35
    
    PULL Strength
    35
    
    VELOCITY (Trip Point)
    35
  - Step-By-Step Patch Pipette Programming
    35
  - Technical Tips for Pulling Micropipettes
    37
    
    Air Flow
    37
    
    DELAY Mode of Active Cooling
    37
    
    Filament Width
    37
    
    Pipette Position
    37
    
    Regulating the Length and Tip Size of a Sharp Pipette
    37
    
    Regulating the Time It Takes to Pull a Sharp Pipette
    37
  - Fire Polishing
    38
  - Figure 3-10. Side View of Fire Polishing Spacer
    38
- Heating Filaments
  39
  - General Information
    39
  - Filament Positioning and Aligning
    39
  - Figure 3-11. Fire Polish Spacer in Puller Bar
    39
  - Figure 3-12. Filament Alignment
    39
  - Box Filament
    40
    
    Figure 3-13. End View of Box Filament and Glass
    40
    
    Positioning
    40
    
    Geometry
    41
  - Trough Filament
    41
    
    Figure 3-14. Box Filament Positioning
    41
    
    Figure 3-15. End View of Trough Filament and Glass
    41
    
    Table 3-4. Box Filament Sizes
    41
    
    Figure 3-16. Trough Filament Positioning
    42
    
    Geometry
    42
    
    Positioning
    42
    
    Table 3-5. Trough Filament Sizes
    42
4 Maintenance

43
5 Troubleshooting

49
APPENDIX A. Limited Warranty

53
APPENDIX B. Accessories

55
- Filaments
  55
- Capillary Glass Tubing
  55
- Other Accessories
  55
APPENDIX C. Fuse Replacement

57
- Figure 5-1. Power Entry Module
  57
- Figure 5-2. Fuse Holder
  58
APPENDIX D. Technical Specifications

59
APPENDIX E. Drierite Material Safety Data Sheet

61
- Index
  63

Sutter Instrument P-97 Operation Manual (62 pages)

Flaming/Brown Micropipette Puller

Brand: Sutter Instrument | Category: Laboratory Equipment | Size: 0 MB

Table of Contents
3
General Information
5
Technical Support
5
What ' S New on the P-97
5
Safety Warning
7
Precautions
8
On Operation
8
On Handling Micropipettes
8
Glass Capillary & Heating Filament Specifications
9
Unpacking
10
Warranty Registration
10
Setting up
11
Line Power (Mains)
11
First Time Use
12
Front Panel
14
Controls
14
Display
15
Write Protection
15
Pressure Setting
15
Mechanical Description (Puller Anatomy)
17
Some Basic Information
17
Air Cooling System
17
Heating Assembly
18
Filament Block Assembly
18
Upper Cable Pulley Assembly
19
Cabinet
20
Base Plate
20
Electronics
20
Programs
21
Program Structure
21
Cycle Parameters
21
Pull Cycle
23
Default Configuration
24
Selecting a Program
24
Viewing a Program
25
Clearing a Program from Memory
25
Editing a Program
26
Entering a New Program
26
Editing an Existing Program
26
Software Control Functions
27
Write-Protect the Program < 7
27
Ramp Test < 1
28
Change Air Pressure < 2
29
Air Mode < 3
29
Time Mode
29
Delay Mode
29
Air Time at Start of Pull < 4
30
Air Time at End of Pull < 5
30
Reset Time and Date < 6
30
Memory Test < 8
30
Pulling Pipettes
31
Procedures
31
Notes on Program Operation
32
Parameter Adjustment
33
General Information
33
Micropipette/Microinjection Needle Fabrication
33
Pull Strength
33
VELOCITY (Trip Point)
34
Delay Mode of Active Cooling
34
Pressure Adjustment
35
Filament Width
35
Patch Pipette Fabrication
36
Technical Tips for Pulling Micropipettes
39
Fire Polishing
40
Heating Filaments
42
General Information
42
Trough Filament
42
Box Filament
43
Maintenance
45
Cleaning
45
Heating Filament Replacement
45
Pulley Adjustment
47
Regeneration of Drierite Granules
48
Troubleshooting
50
Controlling Pipette Tip Shapes
50
Problem: ONE ELECTRODE IS MUCH LONGER than the OTHER ELECTRODE
52
Controller Problems
52
Problem: DISPLAY BLANK, FAN NOT on
53
Problem: DISPLAYED PROGRAM VALUES ARE NOT CORRECT
53
Technical Support
53
Fuse Replacement
54
Power Entry Module
54
Spare Fuse
54
Fuse Holder
54
Limited Warranty
55
Disclaimer
56
Technical Specifications
57
Appendix: Adrierite Material Safety Data Sheet
58
Section VII Spill or Leak Procedures
59
Section VIII Control Measures
59
Index
60

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Sutter Instrument P-97 Manuals

Sutter Instrument P-97 Operation Manuals (66 pages)

Table of Contents

1 General Information

2 Installation

3 Operating Instructions

4 Maintenance

5 Troubleshooting

APPENDIX A. Limited Warranty

APPENDIX B. Accessories

APPENDIX C. Fuse Replacement

APPENDIX D. Technical Specifications

APPENDIX E. Drierite Material Safety Data Sheet

Sutter Instrument P-97 Operation Manual (62 pages)

Table of Contents

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