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Sutter Instrument P-97 Manuals
Manuals and User Guides for Sutter Instrument P-97. We have
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Sutter Instrument P-97 manuals available for free PDF download: Operation Manuals, Operation Manual
Sutter Instrument P-97 Operation Manuals (66 pages)
Flaming/Brown Micropipette Puller
Brand:
Sutter Instrument
| Category:
Laboratory Equipment
| Size: 1 MB
Table of Contents
Disclaimer
5
Safety Warnings and Precautions
5
Electrical
5
Avoiding Electrical Shock and Fire-Related Injury
5
Back Injury Prevention
6
Operational
6
Handling Micropipettes
6
Table of Contents
7
1 General Information
11
What's New on the P-97
11
Glass Capillary & Heating Filament Specifications
12
Glass Capillary
12
Heating Filament
12
Technical Support
12
System Description - Front Panel
12
Figure 1-1. P-97 Front Panel
12
Controls
13
Display
13
Figure 1-2. Program Display
13
System Description - Mechanical (Puller Anatomy)
15
Some Basic Information
15
Air Cooling System
15
Heating Assembly
15
Figure 1-3. P-97 Base Plate (Top View, Cover Removed)
15
Figure 1-4. Filament Block Assembly
16
Upper Pulley Assembly
17
Figure 1-5. Upper Cable Pulley Assembly
17
Cabinet
18
Electronics
18
2 Installation
19
Unpacking
19
Setting up
19
Line Power (Mains)
19
Figure 2-1. P-97 Cabinet (Rear View), Showing Power Entry Module
20
Figure 2-2. P-97 Cabinet (End View, Left), Showing Power Switch
20
Figure 2-3. Power Connection
20
3 Operating Instructions
21
First Time Use
21
Figure 3-1. Loading a Piece of Glass
21
Programs
22
Program Structure
22
Program Line Pull Cycle Parameters
22
Pull Cycle
24
Default Configuration
24
Selecting a Program (0 to 99)
24
Figure 3-2. Power-On Display
24
Table 3-1. Standard Factory Configuration
24
Viewing a Program (< NEXT > / < LAST >)
25
Clearing a Program from Memory (< CLR >)
25
Editing a Program
25
Figure 3-3. P-97 Program Display
25
Figure 3-4. Clear Program Display
25
Editing an Existing Program
26
Entering a New Program
26
Software Control Functions
26
Figure 3-5. Access to Control Functions
26
Air Mode
27
Ramp Test < 1
27
Table 3-2. Control Functions
27
Write-Protect the Program < 7
27
Air Mode < 3
28
Change Air Pressure < 2
28
Table 3-3. Maximum Heat and Recommended Starting Values for Different Filament Shapes
28
Air Time at End of Pull < 5
29
Air Time at Start of Pull < 4
29
Reset Time and Date < 6
29
Memory Test < 8
30
Pulling Pipettes
30
Procedures
30
Figure 3-6. Write Protection Default State
30
Figure 3-7. Pull Cycle Report
30
Notes on Program Operation
31
Parameter Adjustment
31
General Information
31
Figure 3-8. Sample Program
31
Figure 3-9. Pull Cycle Report (Multiple-Loops)
31
Micropipette/Microinjection Needle Fabrication
32
Heat
32
PULL Strength
32
VELOCITY (Trip Point)
32
Delay Mode (Cooling)
33
TIME Mode (Cooling)
33
Filament Width
34
PRESSURE Adjustment
34
Patch Pipette Fabrication
34
Heat
34
Cooling
35
Pressure
35
PULL Strength
35
VELOCITY (Trip Point)
35
Step-By-Step Patch Pipette Programming
35
Technical Tips for Pulling Micropipettes
37
Air Flow
37
DELAY Mode of Active Cooling
37
Filament Width
37
Pipette Position
37
Regulating the Length and Tip Size of a Sharp Pipette
37
Regulating the Time It Takes to Pull a Sharp Pipette
37
Fire Polishing
38
Figure 3-10. Side View of Fire Polishing Spacer
38
Heating Filaments
39
General Information
39
Filament Positioning and Aligning
39
Figure 3-11. Fire Polish Spacer in Puller Bar
39
Figure 3-12. Filament Alignment
39
Box Filament
40
Figure 3-13. End View of Box Filament and Glass
40
Positioning
40
Geometry
41
Trough Filament
41
Figure 3-14. Box Filament Positioning
41
Figure 3-15. End View of Trough Filament and Glass
41
Table 3-4. Box Filament Sizes
41
Figure 3-16. Trough Filament Positioning
42
Geometry
42
Positioning
42
Table 3-5. Trough Filament Sizes
42
4 Maintenance
43
Cleaning
43
Heating Filament Replacement
43
Filament Replacement (Step by Step)
43
Air Jet Position
43
Positioning the Filament in Relation to the Glass Capillary
43
Figure 4-1. V-Groove Bearings and Puller Bars
43
Figure 4-2. Filament Alignment
44
Pulley Adjustment
45
Figure 4-3. Micropipette Shapes
45
Regeneration of Drierite Granules
46
Removing the Canister
46
Replacing the Granules
46
Figure 4-4. Pulley Adjustment
46
Reinstalling the Canister
47
5 Troubleshooting
49
Controlling Pipette Tip Shapes
49
Problem: What Type of Glass Should be Used
49
Problem: the Resistance of Pulled Pipettes Is too Low. How Can a Higher-Resistance Pipette be Pulled
49
Problem: Okay, but How Can Tips be Made Even Smaller
49
Problem: How Can the Size of a Patch-Pipette be Increased
50
Problem: Patch-Pipette Tips Vary in Size from Pull to Pull
50
Problem: Difficulty Making an Injection Pipette with a 0.5 - 1 MM Tip that also Has a very Short Final Taper and Tip (20 to 50 MM Long). How Can this be Done
50
Problem: the Electrodes Are Bent. How Can They be Made to Pull Straight
50
Problem: One Electrode Is Much Longer than the Other Electrode
50
Problem: the Shape and Resistance of the Pipette Changes from Pull to Pull
51
Controller Problems
51
Problem: the Filament Does Not Light up When Pull Is Pressed
51
Problem: Display Blank, Fan Not On, or Locked Up/Overlap
52
Problem: Display Only Shows a Cursor
52
Problem: Displayed Program Values Are Not Correct
52
Technical Support
52
APPENDIX A. Limited Warranty
53
APPENDIX B. Accessories
55
Filaments
55
Capillary Glass Tubing
55
Other Accessories
55
APPENDIX C. Fuse Replacement
57
Figure 5-1. Power Entry Module
57
Figure 5-2. Fuse Holder
58
APPENDIX D. Technical Specifications
59
APPENDIX E. Drierite Material Safety Data Sheet
61
Index
63
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Sutter Instrument P-97 Operation Manual (62 pages)
Flaming/Brown Micropipette Puller
Brand:
Sutter Instrument
| Category:
Laboratory Equipment
| Size: 0 MB
Table of Contents
Table of Contents
3
General Information
5
Technical Support
5
What ' S New on the P-97
5
Safety Warning
7
Precautions
8
On Operation
8
On Handling Micropipettes
8
Glass Capillary & Heating Filament Specifications
9
Unpacking
10
Warranty Registration
10
Setting up
11
Line Power (Mains)
11
First Time Use
12
Front Panel
14
Controls
14
Display
15
Write Protection
15
Pressure Setting
15
Mechanical Description (Puller Anatomy)
17
Some Basic Information
17
Air Cooling System
17
Heating Assembly
18
Filament Block Assembly
18
Upper Cable Pulley Assembly
19
Cabinet
20
Base Plate
20
Electronics
20
Programs
21
Program Structure
21
Cycle Parameters
21
Pull Cycle
23
Default Configuration
24
Selecting a Program
24
Viewing a Program
25
Clearing a Program from Memory
25
Editing a Program
26
Entering a New Program
26
Editing an Existing Program
26
Software Control Functions
27
Write-Protect the Program < 7
27
Ramp Test < 1
28
Change Air Pressure < 2
29
Air Mode < 3
29
Time Mode
29
Delay Mode
29
Air Time at Start of Pull < 4
30
Air Time at End of Pull < 5
30
Reset Time and Date < 6
30
Memory Test < 8
30
Pulling Pipettes
31
Procedures
31
Notes on Program Operation
32
Parameter Adjustment
33
General Information
33
Micropipette/Microinjection Needle Fabrication
33
Pull Strength
33
VELOCITY (Trip Point)
34
Delay Mode of Active Cooling
34
Pressure Adjustment
35
Filament Width
35
Patch Pipette Fabrication
36
Technical Tips for Pulling Micropipettes
39
Fire Polishing
40
Heating Filaments
42
General Information
42
Trough Filament
42
Box Filament
43
Maintenance
45
Cleaning
45
Heating Filament Replacement
45
Pulley Adjustment
47
Regeneration of Drierite Granules
48
Troubleshooting
50
Controlling Pipette Tip Shapes
50
Problem: ONE ELECTRODE IS MUCH LONGER than the OTHER ELECTRODE
52
Controller Problems
52
Problem: DISPLAY BLANK, FAN NOT on
53
Problem: DISPLAYED PROGRAM VALUES ARE NOT CORRECT
53
Technical Support
53
Fuse Replacement
54
Power Entry Module
54
Spare Fuse
54
Fuse Holder
54
Limited Warranty
55
Disclaimer
56
Technical Specifications
57
Appendix: Adrierite Material Safety Data Sheet
58
Section VII Spill or Leak Procedures
59
Section VIII Control Measures
59
Index
60
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