Sutter Instrument XenoWorks BRM Operation Manual page 34

22
Problem
There is still excessive
vibration at the
micropipette tip during
movement.
XENOWORKS MICROMANIPULATOR SYSTEM (TOUCHDECLUTCH) OPERATION MANUAL – REV.
Cause
4. The O-rings in the
micropipette holder
chuck are missing or
damaged.
5. The micropipette is
dragging on the bottom
or sides of the
microinjection chamber.
1. The micropipette holder
clamp has not been
tightened down
sufficiently.
2. There is too great a
distance between the
micropipette tip and the
point where the
micropipette holder is
gripped by the holder
clamp on the
micromanipulator.
3. The fixing screws of the
mounting adapter are
not tight.
Solution
The micropipette holder has
been designed to minimize
vibration when used with
three O-rings in the chuck,
and when the O-rings are the
only thing contacting the
back end of the micropipette.
Less than three O-rings (or
the back of the micropipette
touching the back of the
holder) may lead to excessive
vibration. Replace all missing
or damaged O-rings.
If the tip is touching the
bottom of the chamber, raise
the micropipette slightly by
turning the Z-axis control
counterclockwise. If the shaft
of the micropipette is
touching the side of the
chamber, consider
repositioning the
micropipette with respect to
the micromanipulator as
described in Section 4.
Tighten the micropipette
holder clamp.
This is a different problem to
the one described in 2 and 3
above. Here there is too much
of the micropipette holder
shaft projecting from the
clamp on the
micromanipulator. Shorten
the micropipette tip-to-clamp
distance by moving the holder
outboard and clamping the
micropipette holder closer to
its tip.
Tighten the fixing screws.
4.10 20141208 ( )