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Sutter Instrument TRIO MP-235 Operation Manual

Three-axis motorized micromanipulator system
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TRIO
™
Three-Axis Motorized
Micromanipulator System
With 25mm X & Y Axes, Tilting 50mm D Axis,
Synthetic Z Axis, and USB Interface
Operation Manual
Rev. 2.23 (20190130) (FW v2.2 & 2.3)
One Digital Drive
Novato, CA 94949
Voice: 415-883-0128 Web: www.sutter.com
Fax:
415-883-0572 Email: info@sutter.com
MP-235

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  • Page 1 TRIO MP-235 ™ Three-Axis Motorized Micromanipulator System With 25mm X & Y Axes, Tilting 50mm D Axis, Synthetic Z Axis, and USB Interface Operation Manual Rev. 2.23 (20190130) (FW v2.2 & 2.3) One Digital Drive Novato, CA 94949 Voice: 415-883-0128 Web: www.sutter.com Fax: 415-883-0572 Email: info@sutter.com...
  • Page 2 Copyright © 2018 Sutter Instrument Company. All Rights Reserved. TRIO™ is a trademark of Sutter Instrument Company. TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)

  • Page 5: Disclaimer

    SAFETY WARNINGS AND PRECAUTIONS Electrical Operate the TRIO MP-235 using 110 – 240 VAC., 50-60 Hz line voltage. This instrument is designed for use in a laboratory environment that has low electrical noise and mechanical vibration. Surge suppression is recommended at all times NOTE: There are no user-replaceable fuses in the TRIO MP-235 system.

  • Page 6: Other

    The micropipettes used with this instrument are very sharp and relatively fragile. Avoid contact with micropipette tips to prevent accidentally impaling oneself. Always dispose of micropipettes by placing them into a well-marked, spill-proof “sharps” container. TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)

  • Page 7: Table Of Contents

    3.3.2 Setting Position for HOME or WORK...................18 3.3.3 Setting the Angle of the Pipette/Headstage Holder .............19 3.3.4 Operating the Virtual Z Axis....................19 3.3.5 Moving to the Home Position (HOME).................19 TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)
  • Page 8 Figure 1-1. The TRIO MP-235 system..................... 9 Figure 2-1. Side view of TRIO MP-235/M showing mounting adapter plate and lock screws..11 Figure 2-2. Mounting the TRIO MP-235/M on the Adapter Plate............12 TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)
  • Page 9 Table 4-4. Get Current Position command....................24 Table 4-5. Move to controller-defined HOME position (‘h’) command..........24 Table 4-6. Move to controller -defined WORK position (‘w’) command..........25 TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)
  • Page 10 Table 3. Config. Switches 6 - 10 (Ver. 2.2 & 2.3+)................32 Table D-4. USB-VCP interface serial port settings................32 Table D-5. Microns/microsteps conversion....................33 Table D-6. Ranges.............................33 Table D-7. TRIO MP-235 external control commands.................34 TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)

  • Page 11: Introduction

    Carefully remove all components from the shipping container. In addition to this manual, the following should be included: TRIO MP-235 ROE Rotary Optical Encoder input device with built-in controller and external power adapter. TRIO MP-235/M electromechanical micromanipulator TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)

  • Page 12: Overview

    TRIO MP-235 system. In the event that you need to transport the TRIO MP-235/M in the future, reapply 2 to 3-inch pieces of masking tape to the same locations. Once the tape has been removed, handle the TRIO MP-235/M with care.

  • Page 13: Installation

    Finally, tapping the PULSE button causes a 3μm advance in the diagonal. This rapid burst of forward motion can assist in sharp electrode cell penetration. All the electronics, except for a small power supply, are housed within the TRIO MP-235 ROE and no separate controller or computer is required.

  • Page 14: Headstage Mounting

    Examine the space of the platform onto which installation is to take place. Attach the control cable to TRIO MP-235/M and move the entire unit around on the platform until the precise desired position is determined. A small bag containing the necessary hardware to attach the TRIO MP-235 to the stand is included.

  • Page 15: Electrical Connections And Initial Operating Instructions

    CAUTION: Unless the TRIO MP-235/M micromanipulator electromechanical baseplate is firmly bolted down to a breadboard or solidly to a firm surface, the TRIO MP-235/M is likely to tip over when fully extending all of its axes, especially if its loaded with a headstage that extends beyond the TRIO MP-235/M’s current center of gravity.

  • Page 16: Roe/Controller Rear Panel Controls And Configuration

    2.5 ROE/Controller Rear Panel Controls and Configuration 2.5.1 Power Switch The power switch for the TRIO MP-235 system is located on the rear panel of the ROE/controller. At power up, the microprocessor in the ROE/controller scans the attached equipment and configures the system accordingly.

  • Page 17: Switch 6: Sensor Test (Firmware < V2.2)

    * Factory default (recommended normal operation setting). ** Use the ON (down) setting for slower speed when the micromanipulator or stage is burdened with heavier loads. TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)

  • Page 18: Switch 8: Reserved (Firmware <= V2.2)

    25mm, so Switch 9 should be set to OFF (up) (factory default for a standard TRIO MP-235 system). For a variant model with 12.5mm of travel in the Y axis, Switch 9 must be set ON (down).

  • Page 19: Operations

    MODES Z-AXIS RELATIVE CONTROL MODE (Synthetic) SPEED & LOCK MODES D-AXIS Y-AXIS CONTROL CONTROL X-AXIS CONTROL Figure 3-1. Front view of the TRIO MP-235 ROE/Controller TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)

  • Page 20: Display

    INSTRUMENT CORP. Figure 3-1. LCD Display showing startup screen. When starting the TRIO MP-235 system for the first time or if the HOME position has not yet been defined (saved), the values of all four axes will be 1,000 micrometers (microns).

  • Page 21: Setting The Angle Of The Pipette/Headstage Holder

    3.3.4 Operating the Virtual Z Axis The TRIO MP-235 consists of three physical axes, X, Y and D. A cosine function utilizing X and D axes and the angle of the holder has been implemented to create a virtual Z-axis. Best results are obtained when using it between 10°...

  • Page 22: Moving To The Work Position (Work)

    Depress RELATIVE for 3 sec. or until beep sounds >= (Text in Blue) Figure 3-10. Relative mode Pressing RELATIVE briefly while in Relative mode, returns displayed coordinates back to Absolute mode TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)

  • Page 23: Mode Indications

    Pulse mode advances the D axis in 2.85 μm steps. Each press of the PULSE button increments the Diagonal axis by one 2.85-μm step beyond the current position. This feature can be used to penetrate tough or resistant tissue. TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)

  • Page 24: External Control

    (www.sutter.com). The TRIO MP-235 requires USB CDM (Combined Driver Model) Version 2.10.00 or higher. The CDM device driver for the TRIO MP-235 consists of two device drivers: 1) USB device driver, and 2) VCP (Virtual COM Port) device driver. Install the USB device driver first, followed by the VCP device driver.

  • Page 25: Axis Position Command Parameters

    (e.g., 2 bytes into 16-bit “word”, or 4 bytes into a 32-bit “long” or “double word”). For the TRIO MP-235, all axis position values (number of microsteps) are stored as “unsigned long” (32-bit) values, and each is transmitted and received to and from the controller as four contiguous bytes.

  • Page 26: Commands

    This command moves to the position defined last defined for the WORK key. Movement is equivalent to pressing the WORK key (X & Y together first, followed by Z, as if moving towards a work position). TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)

  • Page 27: Move To Specified "Home" Position ('H') Command

    Completion indicator 0000 1101 <CR> 4.7.6 Move to Specified X-Axis Position (‘x’ or ‘X’) Command This command moves to a specified position for only the X-axis. TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)

  • Page 28: Move To Specified Y-Axis Position ('Y' Or 'Y') Command

    4. All positions sent and received to and from the controller are in microsteps and consist of 32-bit positive integer values (four contiguous bytes). For C/C++, these are typed as TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)
  • Page 29 In either case, however, an appropriate timeout must be set for the reception of data so that the I/O does not time out before the move is made and/or the delay expires. TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)

  • Page 30: Maintenance

    ROE/Controller cabinet as seen in the figure below and changing cables to appropriate connectors. Figure 6-1. Locations of the axis connectors inside the ROE/Controller TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)

  • Page 31: Appendix A. Limited Warranty

    Warranty work will be performed only at the factory. The cost of shipment both ways is paid for by Sutter Instrument during the first three months this warranty is in effect, after which the cost is the responsibility of the customer.

  • Page 32: Appendix C. Technical Specifications

    Barrel Plug (male)─► (center pin positive) ROE/Controller Cabinet: ◄─Banana male GROUND a ground/earth source │ ─►Alligator clip (1-pin Banana-style (user determined) (hooded) female receptacle) TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)

  • Page 33: Appendix D. Quick Reference

    Down Y Axis Lock Out for Enabled Homing Disabled Down* 1 2 3 4 5 6 7 8 9 10 * Normal operation (factory default). TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)

  • Page 34: External Control

    VCP 255 decimal; 00 – FF hexadecimal, or 00000000 – device driver. 11111111 binary). Each byte in a command TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)

  • Page 35: Table D-5. Microns/Microsteps Conversion

    (including Microsoft Windows and Apple MP-235. Mac OS X). If the platform on which your application is running is Big Endian (e.g., Motorola PowerPC CPU), then TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)

  • Page 36: Table D-6. Ranges

    0121 ‘y’ specified Y (see Ranges table) 0101 1011 0091 ‘Y’ axis Position 1 (4) Y μsteps (‘y’ or ‘Y’) Completion indicator 0000 1101 <CR> TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)
  • Page 37 I/O does not time out before the Little-Endian byte order so byte reordering move is made and/or the delay expires. is not necessary before converting to/from 32-bit “long” values. LabVIEW always TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)
  • Page 38 NOTES TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)

  • Page 39: Index

    Overview............10 virtual COM port (VCP) serial port settings.. 23 Introduction .............9 External-control commands Move to specified..........26 Locking out Y-movement homing....... 15 fuses, mains............33 TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)
  • Page 40 Y-Axis travel length changing rotary knob functions on the ROE. 30 25mm ..............16 RELATIVE button ..........21 Y-axis travel length (firmware v2.2+)....16 NOTES TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)

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