Sutter Instrument P-1000 Manuals

Sutter Instrument P-1000 Manuals

Manuals and User Guides for Sutter Instrument P-1000. We have 1 Sutter Instrument P-1000 manual available for free PDF download: Operation Manual

Sutter Instrument P-1000 Operation Manual

Sutter Instrument P-1000 Operation Manual (104 pages)

Flaming/Brown Micropipette Puller System

Brand: Sutter Instrument | Category: Laboratory Equipment | Size: 4 MB

Table of Contents

Disclaimer
5
Safety Warnings and Precautions
5
1 General Information

13
2 Touchscreen User Interface Guide

21
- Startup Screen
  21
- Figure 2-1. Startup Screen
  21
- Figure 2-2. Startup Fault Report Screen
  21
- Home Screen
  22
  - Opening a Program
    22
  - Calibration of Touch Screen
    22
- Pulling a Pipette
  22
  - Figure 2-3. Home Screen
    22
  - Figure 2-4. Pulling a Pipette
    23
  - Figure 2-5. Pull Report
    24
  - Figure 2-6. View the Pull Report
    24
  - Pull Report
    24
  - View Pull Results
    24
  - Figure 2-7. Ramp Test
    25
  - Figure 2-8. Ramp Test Result
    25
  - Ramp Test
    25
- Menu Screen
  26
  - Set Time & Date
    26
  - Lock Program
    26
  - Preheat Jaws 70°C (Thermolock™)
    26
  - Air before Pull
    26
  - Figure 2-9. Menu Screen
    26
  - Figure 2-10. Setting Time and Date
    26
  - Air after Pull
    27
- Program List
  27
  - Sutter Pipette Cookbook Feature
    27
  - Figure 2-11. Program List
    27
- Editing a Program
  28
- Error Messages During Pull
  33
- Figure 2-19. Edit User Notes
  33
3 Installation

35
- Unpacking
  35
- Setting up
  35
4 Operating Instructions

37
- First Time Use
  37
- Programs
  47
- Menu Screen
  54
  - General
    54
- System Options
  54
- Program Options
  54
- Figure 4-23. Menu Screen
  54
- Ramp Test
  55
  - Figure 4-24. Ramp Test Ramp Value Screen
    55
  - Heat Value Recommendations
    56
- Pulling Pipettes
  56
- Parameter Adjustment
  58
  - General Information
    58
  - Micropipette/Microinjection Needle Fabrication
    58
    
    Heat
    58
    
    PULL Strength
    59
    
    VELOCITY (Trip Point)
    59
    
    TIME Mode (Cooling)
    59
    
    Delay Mode (Cooling)
    60
    
    PRESSURE Adjustment
    60
    
    Filament Width
    60
  - Patch Pipette Fabrication
    60
    
    Heat
    61
    
    PULL Strength
    61
    
    VELOCITY (Trip Point)
    61
    
    Cooling
    61
    
    Pressure
    62
  - Thermolock™ Pre-Heat Mode
    62
  - Step-By-Step Patch Pipette Programming
    65
- Technical Tips for Pulling Micropipettes
  66
  - Regulating the Time It Takes to Pull a Sharp Pipette
    66
    
    Heat
    66
    
    Pipette Position
    67
  - Regulating the Length and Tip Size of a Sharp Pipette
    67
    
    Heat
    67
    
    Filament Width
    67
    
    Air Flow
    67
    
    DELAY Mode of Active Cooling
    67
- Fire Polishing
  68
- Figure 4-28. Side View of Fire Polishing Spacer
  68
- Heating Filaments
  69
  - General Information
    69
  - Filament Positioning and Aligning
    69
  - Figure 4-29. Fire-Polish Spacer in Puller Bar
    69
  - Figure 4-30. Filament Alignment
    69
  - Box Filament
    70
    
    Positioning
    70
    
    Figure 4-31. End View of Box Filament and Glass
    70
    
    Geometry
    71
  - Trough Filament
    71
    
    Figure 4-32. Box Filament Positioning
    71
    
    Figure 4-33. End View of Trough Filament and Glass
    71
    
    Table 4-2. Box Filament Sizes
    71
    
    Figure 4-34. Trough Filament Positioning
    72
    
    Geometry
    72
    
    Positioning
    72
    
    Table 4-3. Trough Filament Sizes
    72
5 Maintenance

73
6 Troubleshooting

79
Appendix A. Limited Warranty

91
Appendix B. Accessories

93
- Filaments
  93
- Capillary Glass Tubing
  93
- Other Accessories
  93
Appendix C. Fuse Replacement

95
- Figure 6-10. Power Entry Module and Fuse Holder
  95
Appendix D. Technical Specifications

97
Appendix E. Drierite Material Safety Data Sheet

99
- Index
  101

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