Sutter Instrument P-2000 Manuals

Sutter Instrument P-2000 Manuals

Manuals and User Guides for Sutter Instrument P-2000. We have 1 Sutter Instrument P-2000 manual available for free PDF download: Operation Manual

Sutter Instrument P-2000 Operation Manual

Sutter Instrument P-2000 Operation Manual (88 pages)

Laser-Based Micropipette Puller System

Brand: Sutter Instrument | Category: Laboratory Equipment | Size: 1 MB

Table of Contents

Disclaimer
5
Laser Safety Warning
5
Safety Warning Labels
6
General Safety Warnings and Precautions
7
1 General Information

15
2 Installation

23
- Unpacking
  23
- Setting up
  23
3 Operations

27
- First Time Use
  27
- Figure 3-1. Left Puller Bar
  27
- Front Panel
  29
- Programs
  32
  - Program Structure
    32
  - Cycle Parameters
    32
  - Table 3-1. FILAMENT Scan Pattern Values
    33
- Pull Cycle
  34
- Software Control Functions
  39
- Pulling Pipettes
  43
  - Procedures
    43
    
    Looping
    43
    
    Figure 3-19. Pull Cycle Report
    43
    
    Figure 3-20. Sample Program
    43
    
    Pull Results
    44
  - Notes on Program Operation
    45
    
    Heat on
    45
    
    Figure 3-21. Pull Cycle Report (Multiple-Loops)
    45
    
    Program Memory
    46
- Parameter Adjustment
  46
  - General Information
    46
  - Micropipette/Microinjection Needle Fabrication
    46
    
    Programs
    46
    
    Selecting Program Parameters
    46
    
    Glass Selection
    48
    
    Glass-Specific Parameter Adjustment
    48
    
    Sample Programs (for Quartz Glass)
    49
  - Patch Pipette Fabrication
    50
    
    Programs
    50
    
    Selecting Program Values
    50
    
    Glass Selection
    51
    
    Glass-Specific Parameter Adjustment
    52
    
    Optical System Adjustments
    52
    
    Suggested Practices
    53
  - Optic Fiber Fabrication
    53
    
    Fiber Preparation
    53
    
    Programs
    53
    
    Selecting Program Parameters
    53
  - Electrospray/Nanospray Tip Fabrication
    54
    
    Glass Preparation
    54
    
    Programs
    54
    
    Selecting Program Parameters
    55
  - Technical Tips
    55
    
    Maintaining Pipette Wall Thickness of Quartz Glass
    55
4 Applications and Techniques

57
5 Maintenance

63
6 Troubleshooting

71
Appendix A. Limited Warranty

75
Appendix B. Accessories

77
- Spacers
  77
- Glass Stops
  77
- Glass Loading Aids
  77
- Pipette Storage Boxes
  77
Appendix C. Fuse Replacement

79
Appendix D. Technical Specifications

81
- Table 6-2. Fuse Type and Rating
  81

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