Sutter Instrument XenoWorks BRM Operation Manual page 10

viii
3.1.6 Cleaning the Micropipette.......................................................................................................17
3.1.7 Changing the Working Range of Movement.........................................................................17
3.1.7.1 Setting a 'Z-Floor'..............................................................................................................17
3.1.8 Exchanging the Micropipette..................................................................................................18
3.2 Starting a Microinjection Session .................................................................................................18
3.2.1 Setting up the Microscope.......................................................................................................18
3.2.2 Setting up the Micromanipulator...........................................................................................18
4. MAINTENANCE...............................................................................................................................21
4.1 General.............................................................................................................................................21
4.2 Troubleshooting ..............................................................................................................................21
4.3 Transporting the Micromanipulator ............................................................................................25
APPENDIX A. LIMITED WARRANTY..............................................................................................27
APPENDIX B. ACCESSORIES............................................................................................................29
Extensions..............................................................................................................................................29
Rotating Base.........................................................................................................................................29
Mounts ...................................................................................................................................................29
Adapters .................................................................................................................................................29
Holders ...................................................................................................................................................29
APPENDIX C. FUSE REPLACEMENT.............................................................................................31
APPENDIX D. TECHNICAL SPECIFICATIONS.............................................................................33
INDEX ....................................................................................................................................................37
TABLE OF FIGURES
Figure 1-1. Components of the XenoWorks micromanipulator. ........................................................... 2
Figure 1-2. Joystick. ................................................................................................................................... 3
Figure 1-3. The rear panel of the joystick. ............................................................................................... 3
Figure 1-4. Joystick height adjustment.................................................................................................... 4
Figure 1-5. Manipulator Mechanical. ....................................................................................................... 6
Figure 1-6. Close up of axis polarity switches.......................................................................................... 7
Figure 2-1. Mounting the right-handed manipulator to the far left corner of the adapter plate. ..... 9
Figure 2-2. Engaging the manipulator base plate in the microscope adaptor. ..................................10
Figure 3-1. Location of LED and declutch touch band.........................................................................13
Figure 3-2. Micropipette alignment controls.........................................................................................14
Figure 3-3 (a and b). Suggested approach for suspended cells with angled micropipettes (ICSI,
Stem Cell, Nuclear Transfer). ...............................................................................................15
Figure 3-4 (a and b). Suggested approach for suspended cells with straight micropipettes
(Pronuclear Injection). ...........................................................................................................15
Figure 3-5 (a and b). Suggested approach for adherent cells rand straight pipettes. .......................15
XENOWORKS MICROMANIPULATOR SYSTEM (TOUCHDECLUTCH) OPERATION MANUAL - REV. 4.10 20141208 ( )