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Sutter Instrument TRIO MP-245A Operation Manual

Three-axis motorized micromanipulator system
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TRIO
MP-245A
™
Three-Axis Motorized
Micromanipulator System
With Synthetic Fourth "D" Axis and
USB Interface for External Control
Operation Manual
Rev. 3.12b (20191001)
One Digital Drive
Novato, CA 94949
Voice: 415-883-0128 Web: www.sutter.com
Fax:
415-883-0572 Email: info@sutter.com

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Summary of Contents for Sutter Instrument TRIO MP-245A

  • Page 1 TRIO MP-245A ™ Three-Axis Motorized Micromanipulator System With Synthetic Fourth “D” Axis and USB Interface for External Control Operation Manual Rev. 3.12b (20191001) One Digital Drive Novato, CA 94949 Voice: 415-883-0128 Web: www.sutter.com Fax: 415-883-0572 Email: info@sutter.com...
  • Page 2 (The picture on the cover page shows a TRIO MP-245A ROE/controller and a TRIO MP-245/M micromanipulator. The Sutter Instrument IPA Headstage shown mounted on the micromanipulator is not included in the TRIO MP-245A Series system.) Copyright © 2019 Sutter Instrument Company. All Rights Reserved.

  • Page 5: Disclaimer

    SAFETY WARNINGS AND PRECAUTIONS Electrical  Operate the TRIO MP-245A using 110 – 240 VAC., 50-60 Hz line voltage. This instrument is designed for use in a laboratory environment that has low electrical noise and mechanical vibration. Surge suppression is always recommended NOTE: There are no user-replaceable fuses in the TRIO MP-245A system.

  • Page 6: Other

    Doing so can result in physical injury (e.g., fingers can be caught and pinched between the moving parts of the micromanipulator). If the TRIO MP-245A system is used in a microinjection environment, please observe  the following. As with most micromanipulation devices, sharp micropipettes can fly out of their holder unexpectedly.

  • Page 7: Table Of Contents

    3.3.3 Setting the Angle of the Pipette/Headstage Holder ............. 20 3.3.4 Operating the Virtual D Axis ....................20 3.3.5 Moving to the Home Position ....................21 3.3.6 Moving to the Work Position ....................21 TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)
  • Page 8 APPENDIX D. QUICK REFERENCE ....................41 D.1. Manual Operation ......................... 41 D.2. Configuration..........................41 Rear-Panel DIP Switches ....................... 41 Internal DIP Switches ........................41 D.3. External Control ........................... 42 INDEX ..............................47 TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)
  • Page 9 Table 4-6. Move to controller -defined WORK position (‘w’) command..........28 Table 4-7. Move to specified “Home” position (‘H’) command............29 Table 4-8. Move to specified “Work” position (‘W’) command............29 TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)
  • Page 10 Table D-4. Microns/microsteps conversion.................... 43 Table D-5. Ranges and bounds........................ 43 Table D-6. TRIO MP-245A external control commands..............43 Table D-7. Straight-Line Move ‘S’ Command Speeds................46 TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)

  • Page 11: Introduction

    TRIO MP-245/M stepper-motor-based electromechanical micromanipulator. This manual consists of four parts: This chapter, Introduction, which provides an overview and general description of the TRIO MP-245A system; Chapter 2, Installation, which describes how to install, set up, and configure all components of the system;...

  • Page 12: Overview

    While the axes provide X and Y orthogonal motion typical of most motorized manipulators, Sutter has introduced a diagonal axis with the TRIO MP-245A so one can move the electrode coaxially at the exact desired angle of approach.
  • Page 13 The TRIO MP-245A’s ROE provides fine control of electrode position and the rate of rotation of ROE dials for each axis determines the speed of travel. The finest step size is less than 100nm. Five conveniently located buttons on the ROE provide control of all the basic functions you will need in normal operation (Work, Home, Lock, Relative, and Pulse).
  • Page 14 (This page intentionally left blank.) TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)

  • Page 15: Installation

    2. INSTALLATION When installing the TRIO MP-245A system for the first time, it is recommended that the components of the system be installed in the following order: TRIO MP-245/M electromechanical micromanipulator first, followed by the TRIO MP-245A/E ROE/Controller. 2.1 Mounting Instructions...

  • Page 16: Headstage Mounting

    TRIO MP-245/M’s current center of gravity. TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)

  • Page 17: Roe/Controller Rear Panel Controls And Configuration

    Upon deciding to directly install the TRIO MP-245A system in your rig, it is useful to follow the initial setup procedure to learn how to move the units to allow easy access to the mounting screws. 1. With the power switch on the back of the ROE in the OFF (0) position, connect the power adapter’s 24VDC cable to the POWER receptacle.

  • Page 18: Rear-Panel Configuration Switches

    Power-off position is retained on power on Homing on Power Enabled: Calibrates to 1,000 µm for all axes on DOWN* power on. Power-off position is forgotten. * Factory default (recommended normal operation setting) TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)

  • Page 19: Internal Configuration Switches

    Internal Switch 3 is unused and reserved for future use. 2.5.1.4 Internal Switch 4 Linear/Nonlinear Manual Operation Internal Switch 4 switches between linear and nonlinear manual operation. Contact Sutter Instrument technical support for more information. TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)
  • Page 20 (This page intentionally left blank.) TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)

  • Page 21: Operations

    Green) Figure 3-1. LCD Display showing startup screen. When starting the TRIO MP-245A system for the first time or if the HOME position has not yet been defined (saved), the values of all four axes will be 1,000 micrometers (microns).

  • Page 22: Control Operations

    3.3.4 Operating the Virtual D Axis The TRIO MP-245A consists of three physical axes, X, Y and Z. A tangent function utilizing X and Z axes and the angle of the holder has been implemented to create a virtual D axis. Use between 10°...

  • Page 23: Moving To The Home Position

    To move to the Work position, press the WORK button. If the current position before pressing WORK is less than the Work position, the movement will be as follows: TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)

  • Page 24: Setting Absolute/Relative Coordinates Mode

    Green) Figure 3-11. Absolute mode 3.3.8 Mode Indications The TRIO MP-245A system has three modes of operation: Absolute coordinates, Relative coordinates, and Lock mode. The display turns color for each specific mode, as shown in the following table. TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)

  • Page 25: Speed Control And Roe Knob Movements (Speed)

    3.4 Micropipette/Headstage Exchange Mounted on the front of the Z-axis of the manipulator is the angle-control plate for the headstage mount. TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)

  • Page 26: Figure 3-12. Angled Side View Of Trio Mp-245/M To Change Headstage Mount

    Make any adjustments needed of the headstage, and then tighten down (but do not over tighten) the lock screw in the center of the holding bracket. TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)

  • Page 27: External Control

    The VCP device driver provides a serial RS-232 I/O interface between a Windows application and the TRIO MP-245A. Although the VCP device driver is optional, its installation is recommended even if it is not going to be used. Once installed, the VCP can be enabled or disabled.

  • Page 28: Command Sequence Formatting

    Little- Endian enforcement may be needed if running on a Big-Endian system. Some processors (e.g., ARM) can be configured for specific endianess. TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)

  • Page 29: Microsteps And Microns (Micrometers)

    The data received consists of fourteen bytes containing X, Y, & Z position (32-bit) values in microsteps (4 bytes each), the angle in degrees (1 byte), and the completion indicator (1 byte). TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)

  • Page 30: Move To Controller-Defined Home Position ('H') Command

    4.7.4 Move to Specified “Home” Position (‘H’) Command This command instructs the controller to move all 3 axes to specified position, moving X & Z Ranges (angle determines order/simultaneity), and Y last (see table). TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)

  • Page 31: Move To Specified "Work" Position ('W') Command

    “Straight-Line Move ‘S’ command is specified with one of sixteen speeds. Actual speed for the can be determined with the following formula: (5000 / 16) * (sp +1), where 5,000 is the TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)

  • Page 32: Interrupt Straight-Line Move ('^C') Command

    <ETX> Command 0D 0000 1101 <CR> Completion indicator 4.7.8 Move to Specified X-Axis Position (‘x’ or ‘X’) Command This command moves to a specified position for only the X-axis. TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)

  • Page 33: Move To Specified Y-Axis Position ('Y' Or 'Y') Command

    1 (4) Z µsteps 0000 1101 <CR> Completion indicator 4.7.11 Setting the Angle (‘A’) Command Sets the angle value, in degrees, to match the angle position of the rotary dovetail TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)

  • Page 34: Recalibrate ('R') Command

    0. The physical Origin position is fixed at beginning of travel (BOT). This means that all higher positions (towards end of travel (EOT)) are positive values; there are no lower positions and therefore no negative values are allowed. TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)
  • Page 35 (5000 / 16) * (sp +1), where 5,000 is the maximum speed in microns/second and “sp” is the speed level 0 (slowest) through 15 (fastest). For mm/second or microns/millisecond, multiply result by 0.001. TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)

  • Page 36: Table 4-16. Straight-Line Move 'S' Command Speeds

    Z or X axis fails to move, causing single- and multi-axis movement commands to fail. The ideal range for smooth movement is 10° to 80°. Factory default is 30°. TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)

  • Page 37: Maintenance

    5. MAINTENANCE Routine cleaning of the TRIO MP-245A system is required to prevent excessive dust accumulations. Wipe all exterior surfaces with a dry, soft, cotton cloth. Periodically inspect all cables and connections to make sure that all connections are made well and that all connectors are well and evenly seated.
  • Page 38 (This page intentionally left blank.) TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)

  • Page 39: Appendix A. Limited Warranty

    Warranty work will be performed only at the factory.  The cost of shipment both ways is paid for by Sutter Instrument during the first three months this warranty is in effect, after which the cost is the responsibility of the customer.

  • Page 40: Appendix B. Accessories

    Tall gantry-stand 10.7 to 15.4 in (27.2 to 39.2 cm) MT-75XT Extra tall gantry-stand 14.7 to 18.5 in (37.4 to 47 cm) Risers can be combined to achieve desired height TRIO MP-245A MICROMANIPULATOR SYSTEM QUICK REFERENCE – REV. 3.12B (20191001) (FW V3.12)

  • Page 41: Appendix C. Technical Specifications

    None replaceable (power protection built into the Power Adapter) Cables (Refer to the following tables for a description of all possible cables.) Table C-1. TRIO MP-245A cables and receptacles/connectors. Controller Rear Panel Port Cable Connector Types Connects to ... Cable Type Cable Max.
  • Page 42 Weight: TRIO MP-245A ROE/controller 2.3 lbs. | 1.04 kg TRIO MP-245/M micromanipulator 3.5 lbs. | 1.6 kg TRIO MP-245A MICROMANIPULATOR SYSTEM QUICK REFERENCE – REV. 3.12B (20191001) (FW V3.12)

  • Page 43: Appendix D. Quick Reference

    *** Switch 3 OFF (up) for MP-845[S]M & MP-245/M; ON (down) for MP-285/M & related devices (3DMS, MT-78, MOM/SOM objective movers). **** Contact Sutter Instrument tech. support for more information. TRIO MP-245 MICROMANIPULATOR SYSTEM EXTERNAL CONTROL QUICK REFERENCE – REV. 3.12B (20191001)

  • Page 44: External Control

    If a command returns data, the last byte If the platform on which your application is running returned is the task-completed indicator. is Big Endian (e.g., Motorola PowerPC CPU), then TRIO MP-245A MICROMANIPULATOR SYSTEM QUICK REFERENCE – REV. 3.12B (20191001) (FW V3.12)

  • Page 45: Table D-4. Microns/Microsteps Conversion

    Moves to the position saved by the HOME controller’s HOME button. X & Z Position (‘h’) move first (angle determines order/-simultaneity), and Y last. 0000 1101 <CR> Completion indicator TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)
  • Page 46 Move Y axis to specified position Ranges specified Y (see table) 0101 1011 0091 ‘Y’ axis Position 1 (4) Y µsteps (‘y’ or ‘Y’) 0000 1101 <CR> Completion indicator TRIO MP-245A MICROMANIPULATOR SYSTEM QUICK REFERENCE – REV. 3.12B (20191001) (FW V3.12)
  • Page 47 TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)

  • Page 48: Table D-7. Straight-Line Move 'S' Command Speeds

    The ideal range for 4.0625 4062.5 4062500 0.159940945 81.25% smooth movement is 10° to 80°. Factory default is 30°. 3.7500 3750.0 3750000 0.147637795 75.00% 3.4375 3437.5 3437500 0.135334646 68.75% NOTES: TRIO MP-245A MICROMANIPULATOR SYSTEM QUICK REFERENCE – REV. 3.12B (20191001) (FW V3.12)

  • Page 49: Index

    ..............34 position value typing ........49 external control external control task-complete indicator ........34 position value bit ordering ....... 49 external control external control TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)
  • Page 50 ROE/Controller controls and configuration moving to the Home Position ...... 23 configuration switches (internal) moving to the Work Position ....... 24 Switch 1 Reserved ........17 pausing Home movements ......25 TRIO MP-245A MICROMANIPULATOR SYSTEM QUICK REFERENCE – REV. 3.12B (20191001) (FW V3.12)
  • Page 51 & precautions operational ............4 SAFETY WARNINGS & PRECAUTIONS ..3 warranty ..............41 electrical ..............3 WORK button (while moving to Work) ....25 Sensor test ............. 17 NOTES: TRIO MP-245A FOUR-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 3.12B (20191001)
  • Page 52 NOTES: TRIO MP-245A MICROMANIPULATOR SYSTEM QUICK REFERENCE – REV. 3.12B (20191001) (FW V3.12)

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