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3.5.2 Load Settings
Figure 3-3 Load tab settings
Pausing at the end of a block allows for buffer changes or
other mechanical adjustments before proceeding with the
next block.
• Wavelength – Type or select the desired wavelength from
190 to 360 nm to be used for UV detection. Note that all
blocks use the same wavelength.
Note
The Equilibrate tab is a "Fluid" block type. See Appendix A.
This block loads the sample onto the column.
The Sample Load tab (Figure 3-3) contains the following:
• Injection – Select Pump to pump liquid sample from the
Sample In ports onto the column. Select Manual to inject
the sample from the manual injection ports (located on
the top of the BioOptix 10) onto the column. Note that
selecting Manual disables the flow rate and volume
settings.
• Flow Rate, mL/min – If a Pump injection is selected, enter
the desired flow rate in mL/min.
• Volume – If a Pump injection is selected, enter the
desired volume in mL.
• Collect Effluent – Select the desired action for effluent
fluid as it comes off the column. If Yes is selected, enter
the desired fraction size.
• Pause at End – Select Yes to have the system wait for
operator intervention after completing this block. Select
No to automatically advance to the next block.
Note
The Load tab is a "Load" block type. See Appendix A.
BioOptix™ 10
Section 3 Operation
3-5
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