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Replacing the lamp
4.5 Troubleshooting
4.5.1 Operating Problems
Problem
Overpressure on one or more channels Sample precipitated (crashed) in column or solid phase cartridge.
No peaks detected
Dripping in unused column positions
System will not prime
To install the new lamp:
Contaminants on the glass bulb will shorten the life span. Oil
from your skin is one such contaminant. Wear clean cotton
gloves when handling the deuterium lamp.
1. Carefully insert the replacement bulb (part number
69-5203-014) into the optical assembly and secure it with
the lamp mounting hardware.
2. Route and secure the wires through the twist lock clamp.
3. Reconnect the wiring connector.
4. Replace the waste port mounting plate and secure it with
the hardware.
Overall performance troubleshooting can be simplified if you
maintain a system log that includes the number and type of
samples that are separated.
If any difficulties or abnormal results are noted, they should be
indicated in the log to assist in diagnosing any system problems.
It is worthwhile to maintain a standard sample that can be run
periodically to check the overall performance of the system.
If you notice in your log that detector responses have changed, or
you are running samples and the absorbance readings are not
what you think they should be, you should run a standard.
If you run a standard sample and obtain the expected results, the
problem is not being caused by the instrument. You can then
examine other causes for your problem, such as the column or
mobile phase.
Table 4-1 Common Problems
Causes
Tubing is kinked.
Injection valve is set at an incorrect position.
Incorrect wavelength.
Lines not purged after last use.
Slight leakage from contaminants in check valves. This is usually not detri-
mental to performance. Column bypass tubes should be used on unused
channels.
Prime all channels to overcome the added minute leakage in each channel.
Make sure the bypass tubes are in place on all channels.
Inlet lines clogged; insufficient filtering.
Inlet lines are not securely connected.
Air leakage at internal fitting.
Section 4 System Maintenance
CAUTION
BioOptix™ 10
4-7
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