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3.6.3 Saving the Experiment
File
3.7 Running the
Experiment
Figure 3-8 BioOptix 10 Operation Buttons
3.7.1 System Operation
Save the experiment by selecting the File>Save menu command.
Use a descriptive file name so that it can be located for later use.
With the experiment file open, some BioOptix 10 operation
buttons along the bottom of the window are active (Figure 3-8).
Buttons will be active or inactive according to the current state of
the system.
Fast
Play
Stop
Forward
Table 3-1 Operation Buttons
Button
Play
Stop
Fast Forward
Next Tube
AutoZero
Show Run Window
To start the experiment, click the Play button. The system begins
operation with the first block and proceeds according to the
experiment and method files.
Referring to Figure 3-7, this first block would be "Equilibrate"
and use the operating parameters specified by the method file
(Figure 3-2). The system would pump 0 %B (100% of buffer A) at
5.0 mL/min. The system would pump 50 mL and then pause
(Figure 3-9). Similarly, the BioOptix 10 would proceed through
the Sample Load, Wash, Elute, and Flush blocks.
Section 3 Operation
Next
Tube
Description
Click to begin the experiment run. If the Stop
button was clicked during the run, clicking the
Play button will resume the run.
Click to suspend the run activity. From this state
you click Play to resume the run, or click Fast
Forward to skip to the next block or abort the
run.
Click the Fast Forward to skip to the next block
or abort the run.
Click this button to advance the fraction collector
to the next row. This is useful when there is a
need to isolate effluent.
The baseline absorbance trace may shift if
buffer solutions are changed mid-run. Click this
button to re-zero the absorbance trace.
Click this button to bring the experiment window
for the run in progress to the foreground. If you
have several other files (e.g. previous experi-
ments or new methods) open in the BioOptix 10
window this button lets you quickly return to the
running experiment.
BioOptix™ 10
3-9
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