Prepare The Pump; How To Deal With Solvents - Agilent Technologies 1260 Infinity II User Manual

Prepare the pump

Purge
Use the Purge function to:
• fill the pump,
• exchange a solvent,
• remove air bubbles in tubes and pump heads.
Seal wash
Seal Wash guarantees a maximum seal life time. Use Seal Wash:
• When using buffers with elevated salt concentrations
• When using volatile solvents with non-volatile additives
Contaminated seal wash solvent
C AU T I O N
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How to deal with solvents

• Use clean bottles only.
• Exchange water-based solvents daily.
• Select solvent volume to be used up within 1 – 2 days.
• Use only HPLC-grade solvents and water filtered through 0.2 μm filters.
• Label bottles correctly with bottle content, and filling date / expiry date.
• Use solvent inlet filters.
• Reduce risk of algae growth: use brown bottles for aqueous solvents, avoid
InfinityLab LC Series 1260 Infinity II Binary Pump User Manual
Do not recycle seal wash solvent to avoid contamination.
Weekly exchange seal wash solvent.
direct sunlight.
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Using the Pump
Best Practices
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